CE–MS-based metabolomics reveals the metabolic profile of maitake mushroom (Grifola frondosa) strains with different cultivation characteristics
| Autor: | Shozo Yoneyama, Yoshihiko Tokuji, Mayumi Sato, Atsuko Miyagi, Maki Kawai-Yamada, Seiki Gisusi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biology Applied Microbiology and Biotechnology Biochemistry Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Species Specificity Chitin Culture Techniques Botany Metabolome Cluster Analysis Metabolomics Food science Molecular Biology Grifola frondosa chemistry.chemical_classification Principal Component Analysis Mushroom Organic Chemistry Electrophoresis Capillary General Medicine Metabolism Amino acid 030104 developmental biology chemistry visual_art visual_art.visual_art_medium Sawdust Grifola Biotechnology Organic acid |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 81:2314-2322 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2017.1387049 |
| Popis: | Maitake mushroom (Grifola frondosa [Dicks.] Gray) is generally cultured using the sawdust of broadleaf trees. The maitake strain Gf433 has high production efficiency, with high-quality of fruiting bodies even when 30% of the birch sawdust on the basal substrate is replaced with conifer sawdust. We performed metabolome analysis to investigate the effect of different cultivation components on the metabolism of Gf433 and Mori52 by performing CE–MS on their fruiting bodies in different cultivation conditions to quantify the levels of amino acids, organic acids, and phosphorylated organic acids. We found that amino acid and organic acid content in Gf433 were not affected by the kind of sawdust. However, Gf433 contained more organic acids and less amino acids than Mori52, and Gf433 also contained more chitin compared with Mori52. We believe that these differences in the metabolome contents of the two strains are related to the high production efficiency of Gf433. |
| Databáze: | OpenAIRE |
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