Machaerium acutifolium compounds with larvicidal activity against Aedes aegypti
Autor: | Fernando Nunes Gouveia, João Paulo Barreto Sousa, Maíra G Sá, Laila S. Espindola, Lais S. Morais, Lorena C Albernaz, Sebastião J Melo, Natália Mg Magalhães |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
Insecticides Ethyl acetate Aedes aegypti Mosquito Vectors 01 natural sciences Median lethal dose chemistry.chemical_compound Aedes Animals Larva Traditional medicine biology Plant Extracts Machaerium acutifolium Fabaceae General Medicine biology.organism_classification Prolonged exposure Plant Leaves 010602 entomology chemistry Insect Science Agronomy and Crop Science Brazil 010606 plant biology & botany |
Zdroj: | Pest management scienceREFERENCES. 77(3) |
ISSN: | 1526-4998 |
Popis: | BACKGROUND Plant extracts and isolated compounds are known for their insecticidal activity. The Aedes aegypti mosquito has a significant medical impact as it transmits a number of arboviruses and is able to develop resistance to the commercially available insecticides. This study investigates larvicidal compounds isolated from Machaerium acutifolium, designated by the Brazilian Forest Service as a sustainable species. RESULTS A M. acutifolium trunk ethyl acetate extract was fractionated using chromatographic methods with full structural elucidation by mass spectrometry (MS), nuclear magnetic resonance and specific rotation analyses revealing: one new 3-arylcoumarin derivative 1; two flavonoids 2 and 3; a trans-stilbene 4, and an unprecedented natural indene 5. The larvicidal activity against Ae. aegypti after 24 h exposure was: crude extract (median lethal dose, LC50 205 mg L-1 ), fraction C (LC50 27 mg L-1 ) and 5 (LC50 24 mg L-1 ). CONCLUSION A M. acutifolium extract showed larvicidal activity, which increased with prolonged exposure, demonstrating LC50 75 mg L-1 after 72 h. Although the flavonoids 2 and 3 and trans-stilbene 4 were deemed inactive according to the adopted mortality limit, additional tests revealed their ability to cause 65% Ae. aegypti larvae mortality, suggesting they could contribute to the larvicidal activity. Compound 5, identified by liquid chromatography-MS, was over eight-fold more toxic to larvae than the crude extract after 24 h. Therefore, 5 constitutes a structural model for new prototypes to control Ae. aegypti. These data reinforce the potential of natural products as a source of commercial alternatives for vector control strategies, respecting both sustainability and eco-friendly principles. © 2020 Society of Chemical Industry. |
Databáze: | OpenAIRE |
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