Complementary Effects of Bifidogenic Growth Stimulators and Ammonium Sulfate in Natural Rubber Serum Powder onBifidobacterium bifidum
| Autor: | Shin Ichi Etoh, Kenji Sonomoto, Ayaaki Ishizaki |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Ammonium sulfate
ved/biology.organism_classification_rank.species Carbohydrates Ultrafiltration Fraction (chemistry) Applied Microbiology and Biotechnology Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Adsorption Natural rubber Molecular Biology Chromatography High Pressure Liquid Bifidobacterium Chromatography Bifidobacterium bifidum biology ved/biology Organic Chemistry Proteins General Medicine biology.organism_classification Stimulation Chemical Culture Media Molecular Weight Vitamin B 12 Ultrafiltration (renal) chemistry Ammonium Sulfate visual_art visual_art.visual_art_medium Rubber Powders Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 63:627-631 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254, which was unable to grow in a fully synthetic medium, B12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cutoff 1000). The active ultrafiltrate was further concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. Through this purification step, it was found that the adsorbed fraction obtained did not stimulate growth independently but acted complementarily with a small amount of ammonium sulfate. The adsorbed fraction was subsequently analyzed on reversed-phase high pressure liquid chromatography, and the activities of the eluates were measured on B12 assay medium with ammonium sulfate. Consequently, it was proved that several peptidic ingredients in the adsorbed fraction increased the growth of B. bifidum. |
| Databáze: | OpenAIRE |
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