Overexpression of miR -155 Promotes Proliferation and Invasion of Human Laryngeal Squamous Cell Carcinoma via Targeting SOCS1 and STAT3

Autor: Hong-jun Liang, Xudong Zhao, Wenyue Ji, Wei Zhang
Rok vydání: 2013
Předmět:
Male
Epidemiology
Cellular differentiation
lcsh:Medicine
Suppressor of Cytokine Signaling Proteins
RNA interference
Molecular cell biology
Cell Movement
Basic Cancer Research
Pathology
lcsh:Science
Regulation of gene expression
Multidisciplinary
Middle Aged
Head and Neck Tumors
Cell biology
Gene Expression Regulation
Neoplastic
Oncology
Gene Knockdown Techniques
Carcinoma
Squamous Cell
Medicine
Female
Cancer Epidemiology
Research Article
STAT3 Transcription Factor
Down-Regulation
Biology
Transfection
miR-155
Head and Neck Squamous Cell Carcinoma
Suppressor of Cytokine Signaling 1 Protein
Downregulation and upregulation
Diagnostic Medicine
microRNA
Genetics
Cancer Genetics
Cancer Detection and Diagnosis
Carcinoma
medicine
Humans
Neoplasm Invasiveness
Laryngeal Neoplasms
Aged
Cell Proliferation
Mucous Membrane
Cell growth
Suppressor of cytokine signaling 1
lcsh:R
Cancers and Neoplasms
medicine.disease
Biomarker Epidemiology
MicroRNAs
Otorhinolaryngology
Head and Neck Cancers
Case-Control Studies
Cancer research
lcsh:Q
Gene expression
Biomarkers
General Pathology
Zdroj: PLoS ONE
PLoS ONE, Vol 8, Iss 2, p e56395 (2013)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0056395
Popis: MicroRNA155 plays an important role in many solid malignancies. Expression and function of miR-155 in laryngeal carcinoma have not been fully understood. This study aims to investigate the expression and function of miR-155 in laryngeal squamous cell carcinoma (LSCC), the relationship between miR-155 and its downstream target suppressor of cytokine signaling 1 (SOCS1)-STAT3 pathway, and the related clinicopathological factors. Sixty-three samples of laryngeal squamous cell carcinoma and twenty-one samples of control mucosa obtained from total laryngectomy cases were analyzed using Western blot analysis and real-time PCR. Hep-2 cells were cultured and transfected with miR-155 mimic and ASO. Cell proliferation, migration and invasion assays were used to determine the role of miR-155 in regulation of LSCC growth, migration, and invasion, respectively. The expression levels of miR-155 in LSCC were significantly higher than those in the control mucosa tissues. Downregulation of SOCS1 expression and elevated expression of STAT3 were also observed in LSCC. The relevance of the three factors were statistically significant. Moreover, knockdown of miR-155 elevated SOCS1expression level, suppressed STAT3 expression, and inhibited hep-2 cells growth, migration and invasion. Whereas overexpression of miR-155 inhibited SOCS1expression, elevated STAT3 expression, and promoted hep-2 cells growth, migration and invasion. Furthermore, the miR-155 levels in T(3) T(4) stages, and poor/moderate cell differentiation were significantly higher than those in T(2) stage and higher degree of cell differentiation. The STAT3 protein in poor/moderate cell differentiation was significantly higher than those in higher degree of cell differentiation. We firstly demonstrated the aberrant expression and function of miR-155 and itsdownstream targets in LSCC. The current findings suggest that miR-155 play promotingrole during the development of LSCC, and miR-155 may be a useful marker for the prognosis and assessment of therapeutic effects.
Databáze: OpenAIRE
Externí odkaz: