Immunoneutralization of Somatostatin, Insulin, and Glucagon Causes Alterations in Islet Cell Secretion in the Isolated Perfused Human Pancreas
Autor: | John H. Walsh, Stefan Moldovan, Watt Pc, Robert Kleinman, Thu-Ha Nguyen, Francis C. Brunicardi, Ronald L. Gingerich |
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Rok vydání: | 2001 |
Předmět: |
Adult
Male endocrine system medicine.medical_specialty Adolescent Somatostatin secretion Endocrinology Diabetes and Metabolism medicine.medical_treatment Biology Models Biological Glucagon Antibodies Islets of Langerhans Endocrinology Internal medicine Insulin Secretion Cadaver Internal Medicine medicine Humans Insulin Secretion Child geography geography.geographical_feature_category Hepatology Glucagon secretion Antibodies Monoclonal Middle Aged Islet Perfusion Glucose Somatostatin Female hormones hormone substitutes and hormone antagonists Hormone |
Zdroj: | Pancreas. 23:302-308 |
ISSN: | 0885-3177 |
Popis: | Introduction In this study, immunoneutralization of endogenous insulin, glucagon, and somatostatin with specific antibodies was used in an isolated perfused human pancreas (IPHP) model. Aims To study intrapancreatic cellular interactions and pancreatic hormonal secretion. Methodology Randomized, sequential 10-minute test intervals of single-pass perfusion with each antibody were performed at 3.9 m M or 11.5 m M steady-state glucose concentrations. Somatostatin, insulin, and glucagon levels were measured in the effluent during basal and immunoneutralization intervals. Results At 3.9 m M glucose concentration, somatostatin antibody (SS-Ab) stimulated insulin and glucagon secretion, insulin antibody (IN-Ab) inhibited glucagon secretion, and glucagon antibody (GN-Ab) stimulated insulin secretion. At 11.5 m M glucose concentration, SS-Ab stimulated insulin secretion, IN-Ab stimulated glucagon and inhibited somatostatin secretion, and GN-Ab stimulated insulin secretion. Conclusion The variation in hormonal responses to immunoneutralization during stimulated and nonstimulated glucose conditions suggests that a dynamic association exists between the pancreatic cells. |
Databáze: | OpenAIRE |
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