ACCUMULATION OF TRYPAN BLUE AND TRYPAN RED IN NEPHROCYTES OF THE FRESH-WATER SHRIMP NEOCARIDINA DENTICULATA (DECAPODA: ATYIDAE)

Autor:	Yoshiko Inoue, Nobuaki Niwa, Masaki Ueno
Rok vydání:	1998
Předmět:	biology Decapoda Vesicle Granule (cell biology) Aquatic Science biology.organism_classification Molecular biology Shrimp chemistry.chemical_compound Pigment chemistry Vital stain visual_art Trypan Red Botany visual_art.visual_art_medium Trypan blue
Zdroj:	Journal of Crustacean Biology. 18:666-672
ISSN:	1937-240X 0278-0372
DOI:	10.1163/193724098x00520
Popis:	Two kinds of vital stains, trypan blue and trypan red, were injected into a small fresh-water shrimp, Neocaridina denticulata. Both stains accumulated in the gills, visible as marked chevron-shaped patterns under the branchiostegite. To examine the metabolism of the injected stains, cytological observations using light and electron microscopes were made. The intake of pigment into the gills was observed immediately after injection, after which it gradually accumulated in the cytoplasm of the nephrocytes. The stains were incorporated into middle-sized vesicles (0.3-1.0-gm diameter) which were fused to tubular structures. These tubular structures connect to each other, and stain precipitates were observed in the lumen of the junctions, strongly suggesting that the middle-sized vesicles are formed from swollen junctions. Middle-sized vesicles grow larger and seem to fuse to form a large vesicle called the central granule. Elimination of the central granule into the blood was not observed, suggesting that the central granule constitutes a final storage site of toxic stains, such as trypan blue or trypan red.
Databáze:	OpenAIRE
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