In vitro comparative cytotoxicity study of a novel biocidal iodo-thiocyanate complex
| Autor: | Aoife Boyd, Vincent O'Flaherty, Lilit Tonoyan, Carol M. Gately, Ruairi Friel, Paul H. Mc Cay, Gerard T.A. Fleming |
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| Přispěvatelé: | Irish Research Council, Enterprise Ireland, School of Natural Sciences, National University of Ireland Galway. |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
FIBROBLASTS Erythrocytes Cell Survival 030106 microbiology Iodine Compounds Library science Toxicology Iodo-thiocyanate complex (ITC) Hemolysis MECHANISMS 03 medical and health sciences chemistry.chemical_compound Irish HUMAN-BLOOD Anti-Infective Agents Political science Escherichia coli Animals Humans Horses Povidone iodine ANTISEPTICS CELL CYTO-TOXICITY LACTOPEROXIDASE Thiocyanate Human blood RANGE General Medicine Hydrogen Peroxide HeLa cytotoxicity Iodides language.human_language 3. Good health 030104 developmental biology In vitro comet assay chemistry DNA-DAMAGE Research council language Lugol's iodine Comet Assay SYSTEM Thiocyanates HeLa Cells |
| Zdroj: | Toxicology in Vitro |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2018.03.014 |
| Popis: | Novel biocides, which avoid the induction of cross-resistance to antibiotics, are an urgent societal requirement. Here, we compared the cytotoxic and bactericidal effects of a new antimicrobial agent, the iodo-thiocyanate complex (ITC), with those of the common antiseptics, hydrogen peroxide (H2O2), povidone iodine (PVP-I) and Lugol's iodine (Lugol). The antimicrobials were co-incubated for 10 min with HeLa and Escherichia coli. cells in the presence and absence of organic matter (Dulbecco's modified Eagle's medium, supplemented with 10% fetal bovine serum). The cytotoxic concentrations of ITC were equivalent to its bactericidal concentrations (7.8 mu g ml(-1)). By contrast, cytotoxic effects of H2O2, PVP-I and Lugol were apparent at concentrations lower than their bactericidal concentrations (250, 250 and 125 mu g ml(-1), respectively). The cellular effects of ITC were not quenched by organic matter, unlike the other antiseptics. ITC, PVP-I and Lugol had hemolytic effect on horse erythrocytes at high concentrations, while H2O2 showed no hemolysis. ITC, at 30 or 300 mu g ml(-1), did not cause DNA breakage in HeLa cells as assessed by an in vitro comet assay in the absence of S9 metabolic activation, whereas H2O2 caused extensive single-strand DNA breaks. The pronounced antimicrobial potency of ITC and its favorable cytotoxicity profile suggests that ITC should be considered for antiseptic applications. This work was funded by the Irish Research Council Government of Ireland Postgraduate Scholarship Scheme (GOIPG/2013/584), by Enterprise Ireland (CF/2011/1605) and School of Natural Sciences at National University of Ireland, Galway. peer-reviewed |
| Databáze: | OpenAIRE |
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