Green tea polyphenols and its constituent epigallocatechin gallate inhibits proliferation of human breast cancer cells in vitro and in vivo
| Autor: | James Warren, Rajesh L. Thangapazham, Jaya P. Gaddipati, Radha K. Maheshwari, Anuj Sharma, Anoop K. Singh |
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| Rok vydání: | 2007 |
| Předmět: |
Cancer Research
Cyclin E GTP' Cyclin D Blotting Western Administration Oral Mice Nude Apoptosis Breast Neoplasms Cell Cycle Proteins Epigallocatechin gallate complex mixtures Catechin Mice chemistry.chemical_compound Phenols Cyclin-dependent kinase Cell Line Tumor Proliferating Cell Nuclear Antigen medicine Animals Anticarcinogenic Agents Humans Cell Proliferation Flavonoids Dose-Response Relationship Drug Tea biology Cell Cycle G1 Phase Mammary Neoplasms Experimental Polyphenols food and beverages Cancer Cell cycle medicine.disease Immunohistochemistry Xenograft Model Antitumor Assays Molecular biology Oncology chemistry Cancer cell biology.protein Cancer research Female Guanosine Triphosphate |
| Zdroj: | Cancer Letters. 245:232-241 |
| ISSN: | 0304-3835 |
| Popis: | Tea [Camellia sinensis (Theaceae)] intake is second only to water in terms of worldwide popularity as a beverage. The Green tea polyphenols have been shown to have a protective effect in prostate cancer in various pre-clinical animal models and has been reported to be effective in several other cancer types as well. An inverse association between the risk of breast cancer and the intake of green tea has also been reported in Asian Americans. Several epidemiological studies have shown that breast cancer progression is delayed in the Asian population that consumes green tea on regular basis. In this study, we report the effectiveness of green tea polyphenols (GTP) and its constituent Epigallocatechin Gallate (EGCG) in tumor regression using both in-vitro cell culture models and in vivo athymic nude mice models of breast cancer. The anti-proliferative effect of GTP and EGCG on the growth of human breast cancer MDA-MB-231 cell was studied using a tetrazolium dye-based (MTT) assay. Both GTP and EGCG treatment had the ability to arrest the cell cycle at G1 phase as assessed by flow cytometry. The expression of Cyclin D, Cyclin E, CDK 4, CDK 1 and PCNA were down regulated over the time in GTP and EGCG treated experimental group, compared to the untreated control group as evaluated by western blot analysis for cell cycle proteins, which corroborated the G1 block. Nude mice inoculated with human breast cancer MDA-MB-231 cells and treated with GTP and EGCG were effective in delaying the tumor incidence as well as reducing the tumor burden when compared to the water fed and similarly handled control. GTP and EGCG treatment were also found to induce apoptosis and inhibit the proliferation when the tumor tissue sections were examined by immunohistochemistry. Our results suggest that GTP and EGCG treatment inhibits proliferation and induce apoptosis of MDA-MB-231 cells in-vitro and in-vivo. All together, these data sustain our contention that GTP and EGCG have anti-tumor properties. |
| Databáze: | OpenAIRE |
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