Evaluation of plasma exosomal miRNAs as potential diagnostic biomarkers of lymph node metastasis in papillary thyroid carcinoma
| Autor: | Zhihui Li, Jingqiang Zhu, Tao Wei, Genpeng Li, Jianyong Lei, Wenjie Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Oncology
medicine.medical_specialty Receiver operating characteristic business.industry Gene Expression Profiling Endocrinology Diabetes and Metabolism Diagnostic marker Lymph node metastasis Thyroid carcinoma MicroRNAs Endocrinology Thyroid Cancer Papillary Lymphatic Metastasis Internal medicine microRNA Biomarkers Tumor medicine Humans Diagnostic biomarker Exosomal mirnas Thyroid Neoplasms DNA microarray business Biomarkers |
| Zdroj: | Endocrine. 75:846-855 |
| ISSN: | 1559-0100 1355-008X |
| DOI: | 10.1007/s12020-021-02949-x |
| Popis: | PURPOSE The early diagnosis of lymph node metastasis (LNM) in papillary thyroid carcinoma (PTC) is clinically important, as it can aid in treatment decision-making and improve prognosis. In the present study, we aimed to identify whether plasma exosomal miRNAs could be potential diagnostic markers of LNM in PTC. METHODS Profiles of plasma exosomal miRNAs were screened using miRNA microarrays. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed in the validation and diagnostic sets to select candidate exosomal miRNAs. Finally, receiver operating characteristic (ROC) curves were generated to evaluate the efficiency of target exosomal miRNAs in distinguishing PTC-N1 patients from PTC-N0 patients. RESULTS In total, 197 miRNAs were found to be differentially expressed in the testing set. Based on the qRT-PCR results, the expression of miR-6774-3p (p |
| Databáze: | OpenAIRE |
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