Astragalus polysacharin inhibits hepatocellular carcinoma-like phenotypes in a murine HCC model through repression of M2 polarization of tumour-associated macrophages
| Autor: | Fengda Zhao, Ya-Ping Lv, Xin-You Pan, Mingyun Ma, Chun Li, Jun Zhao |
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| Rok vydání: | 2021 |
| Předmět: |
proliferation
Pharmaceutical Science Context (language use) RM1-950 migration Invasion Drug Discovery medicine Macrophage Pharmacology biology Chemistry Cell growth tumour growth General Medicine M2 Macrophage medicine.disease biology.organism_classification Phenotype Astragalus Complementary and alternative medicine Mechanism of action Hepatocellular carcinoma Cancer research Molecular Medicine Therapeutics. Pharmacology medicine.symptom Research Article |
| Zdroj: | Pharmaceutical Biology article-version (VoR) Version of Record Pharmaceutical Biology, Vol 59, Iss 1, Pp 1533-1539 (2021) |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.1080/13880209.2021.1991384 |
| Popis: | Context Astragalus polysaccharin (APS), an extract of Astragalus propinquus Schischk, exerts antitumor effects in hepatocellular carcinoma (HCC). Objective This study investigated the mechanism of action of APS in HCC. Materials and methods Tumour-associated macrophages (TAMs) were treated with APS (0, 8, 16 mg/mL) for 24 h. APS (16 mg/mL)-treated TAMs were co-cultured with MHCC97H/Huh7 cells for 24 h. Finally, BALB/c nude mice were divided into PBS, APS (50 mg/kg), APS (100 mg/kg), APS (200 mg/kg) groups: mice were inoculated with Huh7 cells to construct tumour xenograft model, followed by administration of APS (50, 100, 200 mg/kg) or PBS daily for 30 days. Cell proliferation, migration, invasion, tumour growth, macrophage markers and proportions were measured. Results APS 16 mg/mL treatment enhanced the expression of M1 macrophage markers (iNOS, IL-1β and TNF-α) and M1 macrophage proportions, while reducing the expression of M2 macrophage markers (IL-10, Arg-1) and M2 macrophage proportions in TAMs. Moreover, the APS-mediated M1 phenotype of TAMs significantly repressed cell proliferation, migration and invasion of MHCC97H and Huh7 cells. Moreover, APS (50, 100, 200 mg/kg) enhanced M1 macrophage proportions and reduced M2 macrophage proportions in the tumour tissues, and thus inhibited tumour growth of HCC. Discussion and conclusions APS inhibits HCC-like phenotypes in a murine HCC model through repression of M2 polarization of TAMs. This work provides a novel theoretical basis for the application of APS in the clinical treatment of HCC. |
| Databáze: | OpenAIRE |
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