Transcriptomic and functional analyses reveal an antiviral role of autophagy during pepper mild mottle virus infection

Autor:	Xiuxiang Zhao, Yubing Jiao, Xiaodong Li, Yuanhua Wu, Man Yu, Zihao Xia, Mengnan An
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0106 biological sciences 0301 basic medicine Pepper mild mottle virus Nicotiana benthamiana Plant Science 01 natural sciences Transcriptomic analysis Defense response Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Pepper lcsh:Botany Tobacco Autophagy Gene silencing KEGG Gene Plant Diseases biology Sequence Analysis RNA Gene Expression Profiling Tobamovirus fungi food and beverages biology.organism_classification Virology lcsh:QK1-989 Pepper mild mottle virus (PMMoV) 030104 developmental biology Gene Knockdown Techniques Capsicum Research Article 010606 plant biology & botany
Zdroj:	BMC Plant Biology, Vol 20, Iss 1, Pp 1-15 (2020) BMC Plant Biology
ISSN:	1471-2229
Popis:	Background Pepper mild mottle virus (PMMoV) is a member in the genus Tobamovirus and infects mainly solanaceous plants. However, the mechanism of virus-host interactions remains unclear. To explore the responses of pepper plants to PMMoV infection, we analyzed the transcriptomic changes in pepper plants after PMMoV infection using a high-throughput RNA sequencing approach and explored the roles of host autophagy in regulating PMMoV infection. Results A total of 197 differentially expressed genes (DEGs) were obtained after PMMoV infection, including 172 significantly up-regulated genes and 25 down-regulated genes. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that most up-regulated DEGs were involved in plant abiotic and biotic stresses. Further analyses showed the expressions of multiple autophagy-related genes (ATGs) were increased after PMMoV infection in pepper and Nicotiana benthamiana plants. Through confocal microscopy and transmission electron microscopy, we have found that PMMoV infection in plant can induce autophagy, evidenced by the increased number of GFP-ATG8a fluorescent punctate and the appearance of double membrane autophagic structures in cells of N. benthamiana. Additionally, inhibition of autophagy significantly increased PMMoV RNA accumulation and aggravated systemic PMMoV symptoms through autophagy inhibitor (3-MA and E64d) treatment and silencing of NbATG expressions by a Tobacco rattle virus-induced gene silencing assays. These results indicated that autophagy played a positive role in plant resistance to PMMoV infection. Conclusions Taken together, our results provide a transcriptomic insight into pepper responding to PMMoV infection and reveal that autophagy induced by PMMoV infection has an antiviral role in regulating PMMoV infection. These results also help us to better understand the mechanism controlling PMMoV infection in plants and to develop better strategies for breeding projects for virus-resistant crops.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e68167917592e221aab63c74f92301d7 http://link.springer.com/article/10.1186/s12870-020-02711-x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.