CX3CR1-fractalkine axis drives kinetic changes of monocytes in fibrotic interstitial lung diseases
| Autor: | Oliver Eickelberg, Flavia R. Greiffo, Isis E. Fernandez, Anne Hilgendorff, Almudena Ortega-Gomez, Valeria Viteri-Alvarez, Daniela Dietel, Marion Frankenberger, Jürgen Behr, Oliver Soehnlein, Joyce S. Lee |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty CX3C Chemokine Receptor 1 CCL2 behavioral disciplines and activities Monocytes Flow cytometry 03 medical and health sciences 0302 clinical medicine CX3CR1 medicine Humans CX3CL1 Lung medicine.diagnostic_test business.industry Chemokine CX3CL1 Monocyte Interstitial lung disease Endothelial Cells respiratory system medicine.disease Flow Cytometry respiratory tract diseases body regions 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis business Lung Diseases Interstitial Hypersensitivity pneumonitis |
| Zdroj: | Eur. Respir. J. 55:1900460 (2020) |
| ISSN: | 1399-3003 |
| Popis: | Circulating immune cell populations have been shown to contribute to interstitial lung disease (ILD). In this study, we analysed circulating and lung resident monocyte populations, and assessed their phenotype and recruitment from the blood to the lung in ILD. Flow cytometry analysis of blood samples for quantifying circulating monocytes was performed in 105 subjects: 83 with ILD (n=36, n=28 and n=19 for nonspecific interstitial pneumonia, hypersensitivity pneumonitis and connective-tissue disease-associated ILD, respectively), as well as 22 controls. Monocyte localisation and abundance were assessed using immunofluorescence and flow cytometry of lung tissue. Monocyte populations were cultured either alone or with endothelial cells to assess fractalkine-dependent transmigration pattern. We show that circulating classical monocytes (CM) were increased in ILD compared with controls, while nonclassical monocytes (NCM) were decreased. CM abundance correlated inversely with lung function, while NCM abundance correlated positively. Both CCL2 and CX3CL1 concentrations were increased in plasma and lungs of ILD patients. Fractalkine co-localised with ciliated bronchial epithelial cells, thereby creating a chemoattractant gradient towards the lung. Fractalkine enhanced endothelial transmigration of NCM in ILD samples only. Immunofluorescence, as well as flow cytometry, showed an increased presence of NCM in fibrotic niches in ILD lungs. Moreover, NCM in the ILD lungs expressed increased CX3CR1, M2-like and phagocytic markers. Taken together, our data support that in ILD, fractalkine drives the migration of CX3CR1+ NCM to the lungs, thereby perpetuating the local fibrotic process. |
| Databáze: | OpenAIRE |
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