The anxiolytics CI-988 and chlordiazepoxide fail to reduce immediate early gene mRNA stimulation following exposure to the rat elevated X-maze
| Autor: | Pierre Brown, Gillian L. Hinks, John Hughes, J.A. Poat, Mark J. Field |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Transcriptional Activation medicine.medical_specialty Indoles medicine.drug_class Stimulation Biology Anxiolytic Chlordiazepoxide CI-988 Meglumine Internal medicine Gene expression medicine Animals RNA Messenger Northern blot Genes Immediate-Early Pharmacology Benzodiazepine Behavior Animal Rats Endocrinology Anti-Anxiety Agents Gene Expression Regulation Immediate early gene medicine.drug |
| Zdroj: | European Journal of Pharmacology. 312:153-161 |
| ISSN: | 0014-2999 |
| DOI: | 10.1016/0014-2999(96)00471-2 |
| Popis: | This study uses immediate early transcription factor gene expression to map neuronal activation after a single exposure to the elevated X-maze. Exposure to this novel environment leads to widespread upregulation in the gene expression of c-fos, NGFI-A and NGFI-B (the nerve growth factor induced genes), but not c-jun nor jun B as shown by in situ hybridization and northern blot analysis. Changes in c-fos were evident after just 5 min exposure to the maze. The cholecystokininB receptor antagonist, CI-988, given intraperitoneally at 1 mg/kg 40 min prior to exposure to the X-maze demonstrated an anxiolytic profile without affecting overall movement around the maze, however it did not reduce the increased levels of gene expression with the methodology used. Likewise the anxiolytic benzodiazepine, chlordiazepoxide at 3 mg/kg did not reduce gene expression. It is concluded that a reduction in an index of behavioural stress/anxiety produced by anxiolytic agents is not concomitantly followed by a detectable reduction in immediate early gene induction. |
| Databáze: | OpenAIRE |
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