The influence of an alternate template conformation on elongating phage T7 RNA polymerase
| Autor: | Peter Dröge, Fritz M. Pohl |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic RNA-dependent RNA polymerase RNA polymerase II Abortive initiation Viral Proteins chemistry.chemical_compound Polydeoxyribonucleotides RNA polymerase Genetics medicine RNA polymerase I T7 RNA polymerase Promoter Regions Genetic Polymerase Transcription bubble biology DNA Superhelical Antibodies Monoclonal DNA-Directed RNA Polymerases Molecular biology Cell biology Kinetics chemistry DNA Viral biology.protein Nucleic Acid Conformation T-Phages medicine.drug |
| Zdroj: | Nucleic Acids Research. 19:5301-5306 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/19.19.5301 |
| Popis: | We investigated the effect of left-handed Z-DNA on transcription by bacteriophage T7 RNA polymerase in vitro and, surprisingly, found that the enzyme can efficiently utilize a template containing a stretch of left-handed DNA close to the promoter. Analysis of transcription products revealed that only a small fraction of elongating polymerases abort transcription either at the promoter proximal or at the distal B-to-Z junction and, even less frequently, within the stretch of left-handed DNA. Our results indicate that, unlike E. coli RNA polymerase, T7 RNA polymerase can utilize a template with a CG stretch in an alternate conformation. In contrast, polymerases are completely blocked at the promoter proximal junction by a monoclonal antibody directed against Z-DNA. This blockage remains stable over a remarkable time, even when negative supercoiling is released by linearization of the template. Together with our recent finding of transcription-induced formation of Z-DNA (3), our data provide an example for a possible auto-regulatory mechanism that employs a change in DNA conformation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|