Quantitative and structural analyses of the in vitro and ex vivo biofilm-forming ability of dermatophytes
| Autor: | Marcos Fábio Gadelha Rocha, Adriana de Queiroz Pinheiro, Lúcio Jackson Queiroz Chaves, Raimunda Sâmia Nogueira Brilhante, Edmilson Emanuel Monteiro Correia, José Júlio Costa Sidrim, Jonathas Sales de Oliveira, Débora de Souza Collares Maia Castelo-Branco, Glaucia Morgana de Melo Guedes, Rossana de Aguiar Cordeiro, Silviane Praciano Bandeira, Waldemiro de Aquino Pereira Neto, Ana Raquel Colares de Andrade, Vandbergue Santos Pereira, Lucas Pereira de Alencar |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Microsporum gypseum Trichophyton rubrum medicine.disease_cause Microbiology 03 medical and health sciences Trichophyton medicine Humans Microsporum Microsporum canis Trichophyton tonsurans Microscopy Staining and Labeling biology Biofilm General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Staining 030104 developmental biology Nails Biofilms Dermatophyte |
| Zdroj: | Journal of Medical Microbiology. 66:1045-1052 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Purpose. The aim of this study was to evaluate the in vitro and ex vivo biofilm-forming ability of dermatophytes on a nail fragment. Methodology. Initially, four isolates of Trichophyton rubrum, six of Trichophyton tonsurans, three of Trichophyton mentagrophytes, ten of Microsporum canis and three of Microsporum gypseum were tested for production biomass by crystal violet assay. Then, one strain per species presenting the best biofilm production was chosen for further studies by optical microscopy (Congo red staining), confocal laser scanning (LIVE/DEAD staining) and scanning electron (secondary electron) microscopy. Results. Biomass quantification by crystal violet assay, optical microscope images of Congo red staining, confocal microscope and scanning electron microscope images revealed that all species studied are able to form biofilms both in vitro and ex vivo, with variable density and architecture. M. gypseum, T. rubrum and T. tonsurans produced robust biofilms, with abundant matrix and biomass, while M. canis produced the weakest biofilms compared to other species. Conclusion. This study sheds light on biofilms of different dermatophyte species, which will contribute to a better understanding of the pathophysiology of dermatophytosis. Further studies of this type are necessary to investigate the processes involved in the formation and composition of dermatophyte biofilms. |
| Databáze: | OpenAIRE |
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