Noninvasive High-Speed Photoacoustic Tomography of Cerebral Hemodynamics in Awake-Moving Rats
| Autor: | Paul R. Carney, Jianbo Tang, Junli Zhou, Lei Xi, Hua Huang, Tao Zhang, Huabei Jiang |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology medicine.medical_specialty Hemodynamics Hyperoxia GABA Antagonists Photoacoustic Techniques Rats Sprague-Dawley Neuroimaging In vivo medicine Animals Wakefulness Pentylenetetrazol business.industry Feature Article Rats Functional imaging Neurology Cerebral hemodynamics Cerebrovascular Circulation Pentylenetetrazole Neurology (clinical) medicine.symptom Cardiology and Cardiovascular Medicine business Blood Flow Velocity Locomotion Feature Article Commentary medicine.drug Biomedical engineering |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism. 35:1224-1232 |
| ISSN: | 1559-7016 0271-678X |
| DOI: | 10.1038/jcbfm.2015.138 |
| Popis: | We present a noninvasive method of photoacoustic tomography (PAT) for imaging cerebral hemodynamics in awake-moving rats. The wearable PAT (wPAT) system has a size of 15 mm in height and 33 mm in diameter, and a weight of ~ 8 g (excluding cabling). The wPAT achieved an imaging rate of 3.33 frames/s with a lateral resolution of 243 μm. Animal experiments were designed to show wPAT feasibility for imaging cerebral hemodynamics on awake-moving animals. Results showed that the cerebral oxy-hemoglobin and deoxy-hemoglobin changed significantly in response to hyperoxia; and, after the injection of pentylenetetrazol (PTZ), cerebral blood volume changed faster over time and larger in amplitude for rats in awake-moving state compared with rats under anesthesia. By providing a light-weight, high-resolution technology for in vivo monitoring of cerebral hemodynamics in awake-behaving animals, it will be possible to develop a comprehensive understanding on how activity alters hemodynamics in normal and diseased states. |
| Databáze: | OpenAIRE |
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