AAV-mediated chronic over-expression of SNAP-25 in adult rat dorsal hippocampus impairs memory-associated synaptic plasticity
| Autor: | Niamh C. O’Sullivan, Jennifer S. Loscher, Olive McCabe, Graham K. Sheridan, Menelas N. Pangalos, Alfonso Blanco Fernández, John J. O'Connor, Mary Moran, Keith J. Murphy, Arpad Palfi, Ciaran M. Regan, Laura Batti, Alex G. McKee, Naomi Chadderton, William T. O'Connor, G. Jane Farrar, Peter Humphries, Sean D. O'Shea |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Receptor complex Synaptosomal-Associated Protein 25 Microdialysis Conditioning Classical Green Fluorescent Proteins Neural facilitation Biophysics Hippocampus Glutamic Acid Neurotransmission In Vitro Techniques Transfection Biochemistry Cellular and Molecular Neuroscience chemistry.chemical_compound Transduction Genetic Avoidance Learning Animals Humans Fear conditioning Rats Wistar Neurotransmitter Maze Learning Cell Line Transformed Memory Disorders Neuronal Plasticity Neural Inhibition Dependovirus Flow Cytometry Electric Stimulation Rats Disease Models Animal chemistry Gene Expression Regulation Synaptic plasticity Exploratory Behavior Memory consolidation Psychology Neuroscience |
| Zdroj: | Journal of neurochemistry. 112(4) |
| ISSN: | 1471-4159 |
| Popis: | Long-term memory is formed by alterations in glutamate-dependent excitatory synaptic transmission, which is in turn regulated by synaptosomal protein of 25 kDa (SNAP-25), a key component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex essential for exocytosis of neurotransmitter-filled synaptic vesicles. Both reduced and excessive SNAP-25 activity has been implicated in various disease states that involve cognitive dysfunctions such as attention deficit hyperactivity disorder, schizophrenia and Alzheimer's disease. Here, we over-express SNAP-25 in the adult rat dorsal hippocampus by infusion of a recombinant adeno-associated virus vector, to evaluate the consequence of late adolescent-adult dysfunction of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein in the absence of developmental disruption. We report a specific and significant increase in the levels of extracellular glutamate detectable by microdialysis and a reduction in paired-pulse facilitation in the hippocampus. In addition, SNAP-25 over-expression produced cognitive deficits, delaying acquisition of a spatial map in the water maze and impairing contextual fear conditioning, both tasks known to be dorsal hippocampal dependent. The high background transmission state and pre-synaptic dysfunction likely result in interference with requisite synapse selection during spatial and fear memory consolidation. Together these studies provide the first evidence that excess SNAP-25 activity, restricted to the adult period, is sufficient to mediate significant deficits in the memory formation process. |
| Databáze: | OpenAIRE |
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