Evaluation of an in vitro fibre fermentation method using feline faecal inocula : inter-individual variation
| Autor: | Bosch, Guido, Heesen, Lisa, de Melo Santos, Karine, Cone, John W, Pellikaan, Wilbert F, Hendriks, Wouter H, LS Voeding, dFAH AVR |
|---|---|
| Přispěvatelé: | LS Voeding, dFAH AVR |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
WM wheat middlings Animal Nutrition Endocrinology Diabetes and Metabolism Butyrate engineering.material 03 medical and health sciences Gas production Tmax time at which Rmax occurred Organic matter Food science Incubation Wheat middlings chemistry.chemical_classification Nutrition and Dietetics Guar gum biology Pulp (paper) CP citrus pectin digestive oral and skin physiology 0402 animal and dairy science food and beverages In vitro methodology 04 agricultural and veterinary sciences biology.organism_classification SCFA 040201 dairy & animal science Fibre fermentability Diervoeding 030104 developmental biology chemistry FOS fructo-oligosaccharide engineering SBP molassed sugar beet pulp WIAS Cats Fermentation Sugar beet Rmax maximum rate of gas production GG guar gum Food Science Research Article |
| Zdroj: | Journal of Nutritional Science, 6 Journal of Nutritional Science, 6. Cambridge University Press Journal of Nutritional Science Journal of Nutritional Science 6 (2017) |
| ISSN: | 2048-6790 |
| Popis: | The present study aimed to evaluate the inter-individual variability in fermentation of standard fibrous substrates by faecal inocula from ten healthy adult female cats. Substrates were citrus pectin (CP), fructo-oligosaccharides (FOS), guar gum (GG), sugar beet pulp (SBP) and wheat middlings (WM). Each substrate was incubated with faecal inoculum from each cat. Gas production was measured continuously during the 48 h incubation and SCFA and organic matter disappearance (only SBP and WM) after incubation. Out of ten cats, nine produced faeces on the days of inoculum preparation. The substrates contrasted in terms of fermentation parameters measured. The inter-individual variability was in general lower for the more simple and pure substrates (CP, FOS, GG) than for the more complex substrates containing mixtures of fibres (SBP, WM). Furthermore, for total SCFA and gas produced, inter-individual variability was lower than for proportions of butyrate and of branched-chain fatty acids and for the parameters of gas production kinetics. It is concluded that the variability in in vitro fermentation parameters is associated with the complexity of fibrous substrates. The presented data are instrumental for the calculation of number of faecal donors required for precise in vitro characterisation of the fermentability of dietary fibres. In addition, the number of faecal donors should be adjusted to the specific fermentation parameter(s) of interest. |
| Databáze: | OpenAIRE |
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