A mechanism for death receptor discrimination by death adaptors
| Autor: | Cristinel Sandu, Evripidis Gavathiotis, Iga Wegorzewska, Milton H. Werner, Ted Huang |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Fas-Associated Death Domain Protein
Amino Acid Motifs Molecular Sequence Data Apoptosis Biochemistry Jurkat cells Receptors Tumor Necrosis Factor Cell Line Jurkat Cells Protein structure Protein Interaction Mapping Humans FADD Amino Acid Sequence fas Receptor Molecular Biology Death domain Adaptor Proteins Signal Transducing biology Signal transducing adaptor protein Cell Biology Fas receptor TRADD Tumor Necrosis Factor Receptor-Associated Peptides and Proteins Cell biology Protein Structure Tertiary Mutation biology.protein Death effector domain Protein Binding Signal Transduction |
| Zdroj: | The Journal of biological chemistry. 280(36) |
| ISSN: | 0021-9258 |
| Popis: | The death domain and death effector domain are two common motifs that mediate protein-protein interactions between components of cell death signaling complexes. The mechanism by which these domains engage their binding partners has been explored by extensive mutagenesis of two death adaptors, FADD and TRADD, suggesting that a death adaptor can discriminate its intended binding partners from other proteins harboring similar motifs. Death adaptors are found to utilize one of two topologically conserved surfaces for protein-protein interaction, whether that partner is another adaptor or its cognate receptor. These surfaces are topologically related to the interaction between death domains observed in the x-ray crystal structure of the Drosophila adaptor Tube bound to Pelle kinase. Comparing the topology of protein-protein interactions for FADD complexes to TRADD complexes reveals that FADD uses a Tube-like surface in each of its death motifs to engage either CD95 or TRADD. TRADD reverses these roles, employing a Pelle-like surface to interact with either receptor TNFR1 or adaptor FADD. Since death adaptors display a Tube-like or Pelle-like preference for engaging their binding partners, Tube/Pelle-like pairing provides a mechanism for death adaptor discrimination of death receptors. |
| Databáze: | OpenAIRE |
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