The preparation of purified carcinoembryonic antigen of the human digestive system from large quantities of tumor tissue
| Autor: | Phil Gold, Terrance Wilson, John Krupey, S. O. Freedman |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Electrophoresis
Radioimmunoassay Physical Therapy Sports Therapy and Rehabilitation Adenocarcinoma Sepharose Carcinoembryonic antigen Column chromatography Antigens Neoplasm Intestinal Neoplasms Methods Animals Humans Antigens Neoplasm Metastasis Immunoelectrophoresis Chromatography biology Chemistry Goats Liver Neoplasms Rehabilitation Extraction (chemistry) General Medicine Human digestive system Biochemistry Sephadex Yield (chemistry) Chromatography Gel biology.protein Ultracentrifugation |
| Zdroj: | Immunochemistry. 9:617-622 |
| ISSN: | 0019-2791 |
| DOI: | 10.1016/0019-2791(72)90247-9 |
| Popis: | A method is described for the preparation of the carcinoembryonic antigen (CEA) of the human digestive system from large quantities of metastatic tumor tissue. The purification process is far more rapid than that originally described, and results in a higher yield of CEA than that previously obtained. The procedure involves the sequential steps of extraction in perchloric acid, column chromatography on Sepharose 4B and Sephadex G-200, and preparative electrophoresis on Sephadex G-25. The final product shows a high degree of uniformity as determined by both physiochemical and immunochemical criteria. |
| Databáze: | OpenAIRE |
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