Contamination-free and automated composition of a reaction mixture for nucleic acid amplification using a capillary electrophoresis apparatus
| Autor: | Ewald J.B.M. Mensink, Peter C. M. Linssen, A.W.H.M. Kuypers, Henk H Lauer |
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| Rok vydání: | 1998 |
| Předmět: |
Capillary action
Pcr cloning Oligonucleotides Biochemistry Polymerase Chain Reaction Analytical Chemistry Capillary electrophoresis Ethidium Nucleic Acids False Positive Reactions Analysis method Electrophoresis Agar Gel Chromatography Autoanalysis Chemistry Organic Chemistry Mutatie analyse voor de diagnose van ziekten door middel van geautomatiseerde PCR-CE technieken Pipette Electrophoresis Capillary General Medicine DNA Contamination Molecular diagnostics Genes ras Mutation analysis for the diagnosis of human diseases by automated PCR-CE techniques Nucleic acid Indicators and Reagents Drug Contamination |
| Zdroj: | Journal of Chromatography. A, 806, 141-147 Journal of Chromatography. A, 806, 1, pp. 141-147 |
| ISSN: | 0021-9673 |
| Popis: | The acceptance of the polymerase chain reaction (PCR) as an amplification method in molecular diagnostics and the rapid development of capillary electrophoresis (CE) as an analysis method of those PCR products was a reason for us to investigate further integration of those two techniques. Using a fused-silica capillary as a pipette we were able to compose a PCR mixture in the CE apparatus. Because a capillary can be thoroughly rinsed and the CE apparatus is a closed system, the risk of contamination and therefore the occurrence of false positive results is minimized. The fact that a CE system can be fully automated contributes to a more reproducible and standardized PCR composition protocol. |
| Databáze: | OpenAIRE |
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