Functional and probiotic characterization of Ligilactobacillus salivarius CPN60 isolated from calf faeces and its appraisal in rats
| Autor: | Ashok Kumar Pattanaik, Avneesh Kumar, Shalini Sharma, Mokshata Gupta, Asmita Singh, Sunil Ekanath Jadhav, Ashok Verma |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.drug_class Antibiotics Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology law.invention Microbiology Feces chemistry.chemical_compound Probiotic Antibiotic resistance Lactobacillales law RNA Ribosomal 16S medicine Animals Rats Wistar Escherichia coli Gastrointestinal tract biology Probiotics biology.organism_classification Rats Lactic acid chemistry Cattle Bacteria Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 132:575-584 |
| ISSN: | 1389-1723 |
| Popis: | Emerging concern about the emergence of antimicrobial resistance has limited the use of antibiotics in calves. Hence, there is a need to find suitable alternatives to antibiotics to manage gastrointestinal infections in neonatal calves. The objective of the present study was to develop a probiotic of calf-origin for its potential application in calf nutrition. Accordingly, 69 lactic acid bacteria (LAB) strains were isolated from faeces of newborn calves, out of which 10 strains were short-listed for further in vitro testing based on the aggregation time and cell surface hydrophobicity. The results of acid-, bile- and phenol-tolerance tests indicated that out of the ten strains, the isolate CPN60 had better resistance to these adverse conditions likely to be encountered in the gastrointestinal tract. The isolate also showed an optimal ability to produce biofilm. Further assessments reiterated its superiority in terms of co-aggregation and antagonistic activity against pathogenic strains of Escherichia coli. Subsequently, the isolate was identified through 16S rRNA sequencing and sequence homology and designated as Ligilactobacillus salivarius CPN60. The candidate probiotic was evaluated in vivo using 48 male (5 weeks old) Wistar rats, divided into two equal groups viz. control (CON) and probiotic (PRO). During the 4-weeks feeding trial, the PRO group rats were gavaged with one mL culture of L. salivarius CPN60 equivalent to 108 CFU/rat. The in vivo trial results indicated better nutrient utilization efficiency and growth performance (p |
| Databáze: | OpenAIRE |
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