Expression of Toll-like receptor9 in diffuse large B-cell lymphoma: further exploring CpG oligodeoxynucleotide in NFκB pathway
| Autor: | Huei-Chiou Lin, Wan-Ting Huang, Shao-Wen Weng, Jiin-Haur Chuang, Chao-Cheng Huang, Po-Chin Tsai |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Male CpG Oligodeoxynucleotide Apoptosis Biology Pathology and Forensic Medicine Immunophenotyping immune system diseases hemic and lymphatic diseases Cell Line Tumor medicine Immunology and Allergy Humans RNA Messenger Receptor Cell Proliferation B-Lymphocytes Reverse Transcriptase Polymerase Chain Reaction NF-kappa B TLR9 hemic and immune systems General Medicine Middle Aged medicine.disease Antigens CD20 Immunohistochemistry Lymphoma DNA-Binding Proteins CpG site Oligodeoxyribonucleotides Toll-Like Receptor 9 Interferon Regulatory Factors Cancer research Proto-Oncogene Proteins c-bcl-6 Female Signal transduction Diffuse large B-cell lymphoma Signal Transduction |
| Zdroj: | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 120(11) |
| ISSN: | 1600-0463 |
| Popis: | Human Toll-like receptors (TLRs) that recognize a variety of pathogen-associated molecular patterns are associated with activation and immunogenic response in lymphoid neoplasms, but rarely explored in diffuse large B-cell lymphoma (DLBCL). We conducted this study to evaluate the expression of TLR9 in and potential treatment of DLBCL with TLR9 agonist - CpG oligodeoxynucleotide (ODN). The real-time quantitative reverse transcription-polymerase chain reaction was carried out to detect TLR9 expression in 41 formalin-fixed paraffin-embedded samples. The transformation of immunophenotype and NFκB pathway of DLBCL upon CpG ODN stimulation were investigated by a DLBCL cell line. TLR9 was commonly detected in DLBCL with relative mRNA levels above 1.0 × 10(-2) in 35 of 41 cases (85.36%). It was suspected that a high proportion of DLBCL to be activated by CpG stimulation. In vitro study with a DLBCL cell line revealed an increased CD20, but decreased BCL-6 and MUM1/IRF4 expression after treatment with CpG ODN. The NFκB pathway was initially activated, but finally suppressed upon CpG ODN stimulation. The proliferation of tumor cells was also inhibited by long time incubation. These findings provide new insights into the role of TLR9 in DLBCL and potential implication of TLR9 agonist in the treatment of DLBCL. |
| Databáze: | OpenAIRE |
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