Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola
| Autor: | Kazuko Okamoto-Shibayama, Yuki Arai, Eitoyo Kokubu, Yuichiro Kikuchi, Kazuyuki Ishihara, Seikou Shintani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Regulator Virulence Repressor DNA-binding protein Infectious and parasitic diseases RC109-216 Microbiology dentilisin Pathogenesis 03 medical and health sciences 0302 clinical medicine stomatognathic system Gene expression medicine Dentistry (miscellaneous) major surface protein Periodontitis repressor biology Treponema denticola 030206 dentistry biology.organism_classification medicine.disease QR1-502 stomatognathic diseases 030104 developmental biology Infectious Diseases gene expression Original Article Research Article |
| Zdroj: | Journal of Oral Microbiology article-version (VoR) Version of Record Journal of Oral Microbiology, Vol 12, Iss 1 (2020) |
| ISSN: | 2000-2297 |
| Popis: | Objective Treponema denticola is involved in ‘chronic’ periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) and prolyl-phenylalanine specific protease (dentilisin) is yet to be clarified. We determined the gene expression profiles of Msp- and dentilisin-deficient mutants of T. denticola to identify the regulation network of gene expression concomitant with the inactivation of these virulence genes. Methods Gene expression profiles of T. denticola ATCC 35405 (wild type), dentilisin-deficient mutant K1, and msp-deficient mutant DMSP3 were determined using DNA microarray analysis and quantitative real-time reverse transcription PCR (qRT-PCR). Msp and dentilisin protein levels were determined by immunoblotting and proteolytic activity assays. Results In addition to several differentially expressed genes, dentilisin expression was reduced in DMSP3; msp expression was significantly reduced in K1 (p |
| Databáze: | OpenAIRE |
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