Hempseed (Cannabis sativa) Peptides WVSPLAGRT and IGFLIIWV Exert Anti-inflammatory Activity in the LPS-Stimulated Human Hepatic Cell Line
| Autor: | Ivan Cruz-Chamorro, Guillermo Santos-Sánchez, Carlotta Bollati, Martina Bartolomei, Jianqiang Li, Anna Arnoldi, Carmen Lammi |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología, Universidad de Sevilla. CTS160: Neuroendocrinología Molecular., Junta de Andalucía, Ministerio de Educación, Cultura y Deporte |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Inflammation
Lipopolysaccharides Hempseed Anti-Inflammatory Agents NF-kappa B Nitric Oxide Synthase Type II General Chemistry Hydrogen Peroxide Nitric Oxide Article NF-κB Oxidative stress inflammation food peptides Food peptides Hepatocytes oxidative stress Cytokines Humans hempseed General Agricultural and Biological Sciences Cannabis |
| Zdroj: | Journal of Agricultural and Food Chemistry |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | WVSPLAGRT (H2) and IGFLIIWV (H3) are two transepithelial transported intestinal peptides obtained from the hydrolysis of hempseed protein with pepsin, which exert antioxidant activity in HepG2 cells. Notably, both peptides reduce the H2O2-induced reactive oxygen species, lipid peroxidation, and nitric oxide (NO) production levels in HepG2 cells via the modulation of the nuclear factor erythroid 2-related factor 2 and the inducible nitric oxide synthase (iNOS) pathways, respectively. Due to the close link between inflammation and oxidative stress and with the objective of fostering the multifunctional behavior of bioactive peptides, in this study, the molecular characterization of the anti-inflammatory and immunomodulatory properties of H2 and H3 was carried out in HepG2 cells. In fact, both peptides were shown to modulate the production of pro (IFN-γ: −33.0 ± 6.7% H2, p = 0.011; −13.1 ± 2.0% H3, p = |
| Databáze: | OpenAIRE |
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