Deep COI sequencing of standardized benthic samples unveils overlooked diversity of Jordanian coral reefs in the northern Red Sea
| Autor: | Nancy Knowlton, Sydney Rosebraugh, Mamoon M. D. Al-Rshaidat, Matthieu Leray, Allison Snider, Laetitia Plaisance, Amanda Devine, Thomas D. Devine |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biodiversity 010603 evolutionary biology 01 natural sciences DNA barcoding Electron Transport Complex IV 03 medical and health sciences Anthozoa Genetics Animals DNA Barcoding Taxonomic Indian Ocean Molecular Biology Phylogeny geography Jordan geography.geographical_feature_category Geography biology Coral Reefs Ecology High-Throughput Nucleotide Sequencing General Medicine Coral reef biology.organism_classification Indian ocean 030104 developmental biology Benthic zone Dna barcodes Biotechnology |
| Zdroj: | Genome. 59:724-737 |
| ISSN: | 1480-3321 0831-2796 |
| Popis: | High-throughput sequencing (HTS) of DNA barcodes (metabarcoding), particularly when combined with standardized sampling protocols, is one of the most promising approaches for censusing overlooked cryptic invertebrate communities. We present biodiversity estimates based on sequencing of the cytochrome c oxidase subunit 1 (COI) gene for coral reefs of the Gulf of Aqaba, a semi-enclosed system in the northern Red Sea. Samples were obtained from standardized sampling devices (Autonomous Reef Monitoring Structures (ARMS)) deployed for 18 months. DNA barcoding of non-sessile specimens >2 mm revealed 83 OTUs in six phyla, of which only 25% matched a reference sequence in public databases. Metabarcoding of the 2 mm – 500 μm and sessile bulk fractions revealed 1197 OTUs in 15 animal phyla, of which only 4.9% matched reference barcodes. These results highlight the scarcity of COI data for cryptobenthic organisms of the Red Sea. Compared with data obtained using similar methods, our results suggest that Gulf of Aqaba reefs are less diverse than two Pacific coral reefs but much more diverse than an Atlantic oyster reef at a similar latitude. The standardized approaches used here show promise for establishing baseline data on biodiversity, monitoring the impacts of environmental change, and quantifying patterns of diversity at regional and global scales. |
| Databáze: | OpenAIRE |
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