Orchidectomy Increases β-Adrenoceptor Activation-Mediated Neuronal Nitric Oxide and Noradrenaline Release in Rat Mesenteric Artery
| Autor: | Mercedes Ferrer, Ana Sagredo, L. Del Campo, Rosa Aras-López, Javier Blanco-Rivero, Gloria Balfagón |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Adrenergic beta-Antagonists Regulator Endogeny Biology Nitric Oxide Nitric oxide Rats Sprague-Dawley Norepinephrine (medication) Norepinephrine Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Internal medicine Receptors Adrenergic beta medicine Animals Clenbuterol Testosterone Neurotransmitter Neurons Endocrine and Autonomic Systems Adrenergic beta-Agonists Propranolol Electric Stimulation Mesenteric Arteries Rats medicine.anatomical_structure chemistry Vasoconstriction Catecholamine Liberation Orchiectomy medicine.drug Artery |
| Zdroj: | Neuroendocrinology. 84:378-385 |
| ISSN: | 1423-0194 0028-3835 |
| Popis: | Background/Aims: A previous study has demonstrated that endogenous male sex hormones do not alter neuronal nitric oxide (NO) release in rat mesenteric artery. However, the regulatory role of endogenous male sex hormones on noradrenaline (NA) release in rat mesenteric artery is not known. The present study was designed to analyze whether endogenous male sex hormones influence the NA release induced by electrical field stimulation (EFS), as well as the possible modification in NA and neuronal NO release by presynaptic β-adrenoceptor activation. Methods: For this purpose, mesenteric arteries from control and orchidectomized male Sprague-Dawley rats were used. Basal and EFS-induced neuronal NO and NA release, as well as the contractile effect induced by EFS, was measured. Results: Basal and EFS-induced neuronal NO and NA release were similar in arteries from control and orchidectomized rats. The β-adrenoceptor agonist clenbuterol did not modify EFS-induced neuronal NO and NA release in arteries from control rats. In contrast, in arteries from orchidectomized animals, clenbuterol increased both neuronal NO and NA release; this increase was prevented by incubation with the β-adrenoceptor antagonist propranolol. However, the contractile response elicited by EFS was not modified by clenbuterol in either group of rats. Conclusions: These results show that orchidectomy does not alter the EFS-induced NA release. What is more, activation of presynaptic β-adrenoceptors does not modify EFS-induced NA and neuronal NO release in arteries from control rats although it increases the release of both neurotransmitters in arteries from orchidectomized rats. Despite these modifications, the EFS-induced contractile response is preserved in arteries from orchidectomized rats. |
| Databáze: | OpenAIRE |
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