Performance of a commercial serum ELISA for the detection of antibodies to Neospora caninum in whole and skim milk samples
| Autor: | H. Donohue, T.M. Byrem, B.D. Voisinet, Jessica T. Houseman, Paul C. Bartlett |
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| Rok vydání: | 2012 |
| Předmět: |
Veterinary medicine
food.ingredient Antibodies Protozoan Cattle Diseases Enzyme-Linked Immunosorbent Assay Culling Sensitivity and Specificity fluids and secretions Animal science food Neospora Predictive Value of Tests Pregnancy Seroepidemiologic Studies Skimmed milk Animals Humans Lactation Seroprevalence General Veterinary biology Coccidiosis food and beverages General Medicine Reference Standards biology.organism_classification Neospora caninum Milk ROC Curve Pregnancy Complications Parasitic biology.protein Herd Cattle Female Parasitology Reagent Kits Diagnostic Antibody Kappa |
| Zdroj: | Veterinary Parasitology. 190:249-253 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2012.06.005 |
| Popis: | Control of Neospora caninum infection in cattle depends on specific, ante-mortem detection of infected animals and limiting their use as breeding stock or by culling. The objective of the present study was to determine appropriate cut-off values and diagnostic performance of a milk ELISA test using whole and skim milk in a commercial serum ELISA test (IDEXX Neospora Ab). Serum and milk samples were obtained from a total of 475 lactating cows from two herds with and two herds without a previous history of N. caninum-associated abortion. Overall seroprevalence determined by the ELISA was 18.3%. Compared to serum ELISA values, correlation and overall performance assessed by receiver operating characteristic analysis was higher when either whole or skim milk samples were diluted 1:2 compared to undiluted or 1:5 diluted samples. Diagnostic performance for analysis of whole and skim milk was compared at cut-off values that achieved a desired operating characteristic of at least 95% specificity. For whole milk diluted 1:2 and a cut-off of 0.14 (S/P ratio), sensitivity and kappa values were 74.7% (95% CI 64.3-83.4) and 0.70 (95% CI 0.61-0.78), respectively. For skim milk diluted 1:2 and a cut-off of 0.30, sensitivity and kappa values were 77.0% (95% CI 66.8-85.4) and 0.72 (95% CI 0.64-0.80), respectively. Using the selected cut-offs, the IDEXX Neospora Ab Test is equally suited for the analysis of whole and skim milk as a screening tool in neosporosis control programs. |
| Databáze: | OpenAIRE |
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