A novel vaccine delivery system: biodegradable nanoparticles in thermosensitive hydrogel
| Autor: | Xiong Xiao, Qin Jie Wu, Pan Wang, Yongsheng Wang, Da Ke Xiong, Xue Chen Zhu, Chang Yang Gong, Li Yang, Yu Quan Wei |
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| Rok vydání: | 2011 |
| Předmět: |
inorganic chemicals
Clinical Biochemistry Basic fibroblast growth factor Nanoparticle complex mixtures Cancer Vaccines Nanocapsules chemistry.chemical_compound Carcinoma Lewis Lung Mice Endocrinology In vivo Animals Humans health care economics and organizations Chemistry Immunogenicity Vaccination technology industry and agriculture Hydrogels Cell Biology respiratory system In vitro Mice Inbred C57BL Self-healing hydrogels Humoral immunity Biophysics Female Fibroblast Growth Factor 2 |
| Zdroj: | Growth factors (Chur, Switzerland). 29(6) |
| ISSN: | 1029-2292 |
| Popis: | In this work, a novel vaccine delivery system, biodegradable nanoparticles (NPs) in thermosensitive hydrogel, was investigated. Human basic fibroblast growth factor (bFGF)-loaded NPs (bFGF-NPs) were prepared, and then bFGF-NPs were incorporated into thermosensitive hydrogel to form bFGF-NPs in a hydrogel composite (bFGF-NPs/hydrogel). bFGF-NPs/hydrogel was an injectable sol at ambient temperature, but was converted into a non-flowing gel at body temperature. The in vitro release profile showed that bFGF could be released from bFGF-NPs or bFGF-NPs/hydrogel at an extended period, but the release rate of bFGF-NPs/hydrogel was much lower. In vivo experiments suggested that immunogenicity of bFGF improved significantly after being incorporated into the NPs/hydrogel composite, and strong humoral immunity was maintained for longer than 12 weeks. Furthermore, an in vivo protective anti-tumor immunity assay indicated that immunization with bFGF-NPs/hydrogel could induce significant suppression of the growth and metastases of tumors. Thus, the NPs/hydrogel composite may have great potential application as a novel vaccine delivery system. |
| Databáze: | OpenAIRE |
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