A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays
| Autor: | Alexandre Zaragoza, Brian Lam, Julian Lamanna, Darius G. Rackus, Michael D. M. Dryden, Aaron R. Wheeler, Shana O. Kelley |
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| Rok vydání: | 2015 |
| Předmět: |
Immunoassay
Detection limit Materials science Goats Microfluidics Biomedical Engineering Bioengineering Nanotechnology Electrochemical Techniques General Chemistry Antibodies Viral Electrochemistry Biochemistry Nanostructures Indium tin oxide Microelectrode Immunoglobulin G Lab-On-A-Chip Devices Electrode Animals Humans Differential pulse voltammetry Digital microfluidics Microelectrodes Rubella virus |
| Zdroj: | Lab on a Chip. 15:3776-3784 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | Nanostructured microelectrodes (NMEs) are three-dimensional electrodes that have superb sensitivity for electroanalysis. Here we report the integration of NMEs with the versatile fluid-handling system digital microfluidics (DMF), for eventual application to distributed diagnostics outside of the laboratory. In the new methods reported here, indium tin oxide DMF top plates were modified to include Au NMEs as well as counter and pseudoreference electrodes. The new system was observed to outperform planar sensing electrodes of the type that are typically integrated with DMF. A rubella virus (RV) IgG immunoassay was developed to evaluate the diagnostic potential for the new system, relying on magnetic microparticles coated with RV particles and analysis by differential pulse voltammetry. The limit of detection of the assay (0.07 IU mL(-1)) was100× below the World Health Organization defined cut-off for rubella immunity. The sensitivity of the integrated device and its small size suggest future utility for distributed diagnostics. |
| Databáze: | OpenAIRE |
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