Genome-wide transcriptional profiling and enrichment mapping reveal divergent and conserved roles of Sko1 in the Candida albicans osmotic stress response
| Autor: | Leonid Sukala, Jennifer R. Teubl, Dawn H. Marotta, Jason M. Rauceo, André Nantel |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Osmoregulation
Gene Expression Regulation Fungal genetic variability Candida albicans Sko1 gene genetic conservation Promoter Regions Genetic protein hog1 Phylogeny genome analysis Genetics 0303 health sciences biology unclassified drug enzyme activity Mitochondria Complementation Basic-Leucine Zipper Transcription Factors real time polymerase chain reaction Genome Fungal Mitogen-Activated Protein Kinases signal transduction Saccharomyces cerevisiae Proteins Osmotic shock In silico Saccharomyces cerevisiae genetic regulation Article Evolution Molecular Fungal Proteins 03 medical and health sciences Computer Simulation phosphotransferase gene Gene 030304 developmental biology 030306 microbiology Microarray analysis techniques Gene Expression Profiling genetic transcription gene mapping biology.organism_classification DNA binding motif Gene expression profiling Repressor Proteins gene function Vacuolar transport computer model microarray analysis osmotic stress |
| Popis: | Candida albicans maintains both commensal and pathogenic states in humans. Here, we have defined the genomic response to osmotic stress mediated by transcription factor Sko1. We performed microarray analysis of a sko1δ/δ mutant strain subjected to osmotic stress, and we utilized gene sequence enrichment analysis and enrichment mapping to identify Sko1-dependent osmotic stress-response genes. We found that Sko1 regulates distinct gene classes with functions in ribosomal synthesis, mitochondrial function, and vacuolar transport. Our in silico analysis suggests that Sko1 may recognize two unique DNA binding motifs. Our C. albicans genomic analyses and complementation studies in Saccharomyces cerevisiae showed that Sko1 is conserved as a regulator of carbohydrate metabolism, redox metabolism, and glycerol synthesis. Further, our real time-qPCR results showed that osmotic stress-response genes that are dependent on the kinase Hog1 also require Sko1 for full expression. Our findings reveal divergent and conserved aspects of Sko1-dependent osmotic stress signaling. © 2013 Elsevier Inc. |
| Databáze: | OpenAIRE |
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