Legionella dresdenensis sp. nov., isolated from river water
| Autor: | Roger Dumke, Sabine Gronow, Paul Christian Lück, Isolde Röske, Ute Schröter-Bobsin, Enno Jacobs |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Bacilli biology Legionella Molecular Sequence Data Fresh Water General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA rpoB Microbiology Legionella pneumophila chemistry.chemical_compound Bacterial Proteins chemistry RNA Ribosomal 16S Legionella rubrilucens Buffered charcoal yeast extract agar Cysteine Phylogeny Ecology Evolution Behavior and Systematics |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 60:2557-2562 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.017863-0 |
| Popis: | Legionella-like isolates, strains W03-356T, W03-357 and W03-359, from three independent water samples from the river Elbe, Germany, were analysed by using a polyphasic approach. Morphological and biochemical characterization revealed that they were Gram-negative, aerobic, non-spore-forming bacilli with a cut glass colony appearance that grew only on l-cysteine-supplemented buffered charcoal yeast extract agar. Phylogenetic analysis based on sequence comparisons of the 16S rRNA, macrophage infectivity potentiator (mip), gyrase subunit A (gyrA), ribosomal polymerase B (rpoB) and RNase P (rnpB) genes confirmed that the three isolates were distinct from recognized species of the genus Legionella. Phenotypic characterization of strain W03-356T based on fatty acid profiles confirmed that it was closely related to Legionella rubrilucens ATCC 35304T and Legionella pneumophila ATCC 33152T, but distinct from other species of the genus Legionella. Serotyping of the isolates showed that they were distinct from all recognized species of the genus Legionella. Strains W03-356T, W03-357 and W03-359 are thus considered to represent a novel species of the genus Legionella, for which the name Legionella dresdenensis sp. nov. is proposed. The type strain is W03-356T (=DSM 19488T=NCTC 13409T). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|