Measurement uncertainty assessment of magnesium trisilicate column for determination of Sudan colorants in food by HPLC using C8 column
| Autor: | Huang Wenyao, Ying Chen, Song Yi, Ling-feng Dai, Jing-jun Cheng, Chao He, Sheng-wen Shao, Ya-ping Jiang, Jia-fa Liu |
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| Rok vydání: | 2016 |
| Předmět: |
Sudan I
Formic acid Biomedical Engineering chemistry.chemical_element Naphthols 010402 general chemistry 01 natural sciences Biochemistry High-performance liquid chromatography Biomaterials chemistry.chemical_compound Limit of Detection Magnesium Silicates Genetics Acetone Humans Chromatography High Pressure Liquid Earth-Surface Processes Aqueous solution Chromatography Chemistry Magnesium 010401 analytical chemistry Food Coloring Agents 0104 chemical sciences Hexane Measurement uncertainty Azo Compounds Food Analysis |
| Zdroj: | Journal of Huazhong University of Science and Technology [Medical Sciences]. 36:752-757 |
| ISSN: | 1993-1352 1672-0733 |
| Popis: | This study aimed to conduct measurement uncertainty assessment of a new method for determination of Sudan colorants (Sudan I, II, III and IV) in food by high performance liquid chromatography (HPLC). Samples were extracted with organic solvents (hexane, 20% acetone) and first purified by magnesium trisilicate (2MgO·3SiO2). The Sudan colorants (Sudan I-IV) were also initially separated on C8 by gradient elution using acetonitrile and 0.1% (v/v) formic acid aqueous solution as the mobile phases and detected with diode-array detector (DAD). The uncertainty of mathematical model of Sudan I, II, III and IV is based on EURACHEM guidelines. The sources and components of uncertainty were calculated. The experiment gave a good linear relationship over the concentration from 0.4 to 4.0 μg/mL and spiked recoveries were from 74.0% to 97.5%. The limits of determination (LOD) were 48, 61, 36, 58 μg/kg for the four analytes, respectively. The total uncertainty of Sudan colorants (Sudan I, II, III and IV) was 810±30.8, 790±28.4, 750±27.0, 730±50.0 μg/kg, respectively. The recovery uncertainty was the most significant factor contributing to the total uncertainty. The developed method is simple, rapid, and highly sensitive. It can be used for the determination of trace Sudan dyes in food samples. The sources of uncertainty have been identified and uncertainty components have been simplified and considered. |
| Databáze: | OpenAIRE |
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