Ganoderma lucidum Ethanol Extracts Enhance Re-epithelialization and Prevent Keratinocytes from Free-Radical Injury
| Autor: | Manuela Rodriquez, Rosario Randino, Anna Maria D'Ursi, Maurizio Bifulco, Manuela Giovanna Basilicata, Simona Pisanti, Verdiana Covelli, Mario Abate, Giacomo Pepe, Walter Cabri, Pietro Campiglia |
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| Přispěvatelé: | Abate, M., Pepe, G., Randino, R., Pisanti, S., Basilicata, M. G., Covelli, V., Bifulco, M., Cabri, W., D'Ursi, A. M., Campiglia, P., Rodriquez, M., Mario Abate , Giacomo Pepe , Rosario Randino , Simona Pisanti , Manuela Giovanna Basilicata , Verdiana Covelli , Maurizio Bifulco , Walter Cabri , Anna Maria D’Ursi , Pietro Campiglia, Manuela Rodriquez |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway medicine.medical_treatment Pharmaceutical Science lcsh:Medicine lcsh:RS1-441 wound healing Cosmeceuticals Ganoderma lucidum Oxidative stress Triterpenic acids Wound healing Pharmacology cosmeceuticals Article lcsh:Pharmacy and materia medica 03 medical and health sciences 0302 clinical medicine Triterpenic acid Drug Discovery Adaptogen medicine oxidative stress Cytotoxicity Protein kinase B biology Kinase Chemistry Cyclin-dependent kinase 2 lcsh:R Cosmeceutical 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis triterpenic acids biology.protein Molecular Medicine Oxidative stre Keratinocyte |
| Zdroj: | Pharmaceuticals, Vol 13, Iss 224, p 224 (2020) Pharmaceuticals Volume 13 Issue 9 |
| ISSN: | 1424-8247 |
| Popis: | Ganoderma lucidum or Reishi is recognized as the most potent adaptogen present in nature, and its anti-inflammatory, antioxidant, immunomodulatory and anticancer activities are well known. Moreover, lately, there has been an increasing interest from pharmaceutical companies in antiaging G. lucidum-extract-based formulations. Nevertheless, the pharmacological mechanisms of such adaptogenic and regenerative actions remain unclear. The present investigation aimed to explore its molecular and cellular effects in vitro in epidermal keratinocyte cultures by applying liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LCMS-IT-TOF) for analysis of ethanol extracts using ganoderic acid-A as a reference compound. The G. lucidum extract showed a keratinocyte proliferation induction accompanied by an increase of cyclic kinase protein expressions, such as CDK2 and CDK6. Furthermore, a noteworthy migration rate increase and activation of tissue remodelling factors, such as matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), were observed. Finally, the extract showed an antioxidant effect, protecting from H2O2-induced cytotoxicity preventing activation of AKT (protein kinase B), ERK (extracellular signal-regulated kinase), p53 and p21 and reducing the number of apoptotic cells. Our study paves the path for elucidating pharmacological properties of G. lucidum and its potential development as cosmeceutical skin products, providing the first evidence of its capability to accelerate the healing processes enhancing re-epithelialization and to protect cells from free-radical action. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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