Application of the polymerase chain reaction to the detection of African horse sickness viruses
| Autor: | S. Moulay, Stéphan Zientara, A. Wade-Evans, C. Cruciere, Corinne Sailleau |
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| Rok vydání: | 1995 |
| Předmět: |
African Horse Sickness Virus
Molecular Sequence Data Reoviridae Enzyme-Linked Immunosorbent Assay Biology Polymerase Chain Reaction Sensitivity and Specificity Virus law.invention Mice law Virology African Horse Sickness Animals Horses Polymerase chain reaction DNA Primers RNA Double-Stranded Orbivirus Base Sequence RNA RNA-Directed DNA Polymerase biology.organism_classification Reverse transcriptase African horse sickness RNA Viral Spleen |
| Zdroj: | Journal of virological methods. 53(1) |
| ISSN: | 0166-0934 |
| Popis: | The development of a coupled reverse transcriptase-polymerase chain reaction assay (RT-PCR) is described for the detection of African horse sickness virus (AHSV) double-stranded RNA. Genome segments 7 and 10 were chosen as target templates for primers selected for use in the RT-PCR. Using these AHSV-specific primers all 9 serotypes were detectable. The sensitivity and specificity of the RT-PCR results were compared to those obtained by competition ELISA. |
| Databáze: | OpenAIRE |
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