Analysis of a Spontaneous Non-Motile and Avirulent Mutant Shows That FliM Is Required for Full Endoflagella Assembly in Leptospira interrogans
| Autor: | Célia Fontana, Mathieu Picardeau, Nadia Benaroudj, Nathalie Michele Cachet, Olivier Gorgette, David Gasparini, Natalia Bomchil, Ambroise Lambert |
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| Přispěvatelé: | Biologie des Spirochètes / Biology of Spirochetes, Institut Pasteur [Paris], Laboratoire Lyon Gerland, MERIAL, Centre de Recherche Clinique de Saint Vulbas, Microscopie ultrastructurale - Ultrapole (CITECH), This work was funded by the Institut Pasteur (http://www.pasteur.fr), FUI 14 (Fonds Unique Interministériel) 'COVALEPT', BPI France (http://www.bpifrance.fr/), Association Nationale de la Recherche et de la Technologie (http://www.anrt.asso.fr/), and Merial SAS France (http://www.merial.com). The funder provided support in the form of salaries for authors [CF, DG, NC, N. Benaroudj, MP, N. Bomchil, OG, AL] and research materials, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript., Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Pathology and Laboratory Medicine Cell morphology Biochemistry Medicine and Health Sciences lcsh:Science Leptospira Mammals MESH: Gene Expression Regulation Bacterial MESH: Genetic Complementation Test Multidisciplinary Virulence biology Bacterial Pathogens 3. Good health MESH: Leptospira interrogans/ultrastructure Leptospira Interrogans Cell Motility MESH: Leptospira interrogans/physiology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Flagella Medical Microbiology Vertebrates Hamsters Cellular Structures and Organelles Pathogens Leptospira interrogans Research Article Pathogen Motility MESH: Mutation Virulence Factors Motor Proteins 030106 microbiology Motility Flagellum MESH: Bacterial Proteins/genetics Microbiology Rodents Motor protein 03 medical and health sciences Bacterial Proteins Molecular Motors Animals MESH: Virulence/genetics Microbial Pathogens [SDV.GEN]Life Sciences [q-bio]/Genetics Bacteria Genetic Complementation Test lcsh:R Organisms Biology and Life Sciences Proteins Cell Biology Flagellar Motility Gene Expression Regulation Bacterial Periplasmic space MESH: Flagella/physiology biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology 030104 developmental biology MESH: Flagella/ultrastructure Amniotes Mutation lcsh:Q |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2016, 11 (4), pp.e0152916. ⟨10.1371/journal.pone.0152916⟩ PLoS ONE, 2016, 11 (4), pp.e0152916. ⟨10.1371/journal.pone.0152916⟩ PLoS ONE, Vol 11, Iss 4, p e0152916 (2016) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0152916⟩ |
| Popis: | International audience; Pathogenic Leptospira strains are responsible for leptospirosis, a worldwide emerging zoonotic disease. These spirochetes are unique amongst bacteria because of their corkscrew-like cell morphology and their periplasmic flagella. Motility is reported as an important virulence determinant, probably favoring entry and dissemination of pathogenic Leptospira in the host. However, proteins constituting the periplasmic flagella and their role in cell shape, motility and virulence remain poorly described. In this study, we characterized a spontaneous L. interrogans mutant strain lacking motility, correlated with the loss of the characteristic hook-shaped ends, and virulence in the animal model. Whole genome sequencing allowed the identification of one nucleotide deletion in the fliM gene resulting in a premature stop codon, thereby preventing the production of flagellar motor switch protein FliM. Genetic complementation restored cell morphology, motility and virulence comparable to those of wild type cells. Analyses of purified periplasmic flagella revealed a defect in flagella assembly, resulting in shortened flagella compared to the wild type strain. This also correlated with a lower amount of major filament proteins FlaA and FlaB. Altogether, these findings demonstrate that FliM is required for full and correct assembly of the flagella which is essential for motility and virulence. |
| Databáze: | OpenAIRE |
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