A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice
| Autor: | R. A. Greene, E. Conley, M. Trudeau-Spanjers, Jose L. Gonzalez-Hernandez, David Hummel, Bikram S. Gill, Raymond D. Fenton, Henry T. Nguyen, Lili Qi, Venu Kalavacharla, Nora L. V. Lapitan, Khwaja Hossain, J. P. Gustafson, D. W. Choi, Deepak Sidhu, Miftahudin, A. M. Linkiewicz, B. Echalier, J. Dvořák, Muharrem Dilbirligi, Jorge Dubcovsky, Gerard R. Lazo, Calvin O. Qualset, Patrick E. McGuire, Junhua Peng, Vivienne Nduati, James A. Anderson, Xue-Feng Ma, Eduard Akhunov, Mark E. Sorrells, Shiaoman Chao, A. Mesfin, Olin D. Anderson, M. S. Pathan, Timothy J. Close, Shahryar F. Kianian, Kulvinder S. Gill |
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| Rok vydání: | 2004 |
| Předmět: |
Locus (genetics)
Biology Genes Plant Chromosomes Plant Chromosomes Gene density Chromosome regions Chromosome 19 Wheat Investigations Genetics Triticum Synteny Chromosomal inversion Expressed sequence tag Ploidies Genome Human Genome food and beverages Chromosome Mapping Oryza Plant Genes Chromosome 22 Sequence Alignment Genome Plant Developmental Biology |
| Zdroj: | Genetics, vol 168, iss 2 Conley, EJ; Nduati, V; Gonzalez-Hernandez, JL; Mesfin, A; Trudeau-Spanjers, M; Chao, S; et al.(2004). A 2600-locus chromosome bin map of wheat homoeologous group 2 reveals interstitial gene-rich islands and colinearity with rice. Genetics, 168(2), 625-637. doi: 10.1534/genetics.104.034801. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/46q0f25g |
| Popis: | The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7. |
| Databáze: | OpenAIRE |
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