Long-term effects of HTLV-1 on brain astrocytes: Sustained expression of Tax-1 associated with synthesis of inflammatory mediators
| Autor: | Pascale Giraudon, Christine Brisson, Hideo Akaoka, Raphaël Szymocha, Arlette Bernard, Marie-Françoise Belin |
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| Přispěvatelé: | Centre de recherche en neurosciences de Lyon (CRNL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2000 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Retroviridae Proteins Oncogenic MESH: Membrane Glycoproteins Pathogenesis 0302 clinical medicine immune system diseases MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Blotting Southern MESH: Microscopy Confocal MESH: Animals Cells Cultured MESH: Cell Adhesion Molecules Neuronal Human T-lymphotropic virus 1 0303 health sciences Membrane Glycoproteins Microscopy Confocal Reverse Transcriptase Polymerase Chain Reaction virus diseases Interleukin Immunohistochemistry 3. Good health Blotting Southern medicine.anatomical_structure Matrix Metalloproteinase 9 Neurology Matrix Metalloproteinase 2 MESH: Contactin 2 Tumor necrosis factor alpha medicine.symptom MESH: Cells Cultured Astrocyte MESH: Rats Cell Adhesion Molecules Neuronal Inflammation MESH: Microscopy Electron Biology MESH: Coculture Techniques 03 medical and health sciences Cellular and Molecular Neuroscience Virology Contactin 2 medicine Animals Humans RNA Messenger MESH: RNA Messenger 030304 developmental biology MESH: Human T-lymphotropic virus 1 MESH: Humans Tumor Necrosis Factor-alpha MESH: Retroviridae Proteins Oncogenic MESH: Interleukin-1 MESH: Matrix Metalloproteinase 9 MESH: Immunohistochemistry biology.organism_classification Coculture Techniques Rats MESH: Astrocytes MESH: Matrix Metalloproteinase 2 Microscopy Electron CTL Viral replication Astrocytes MESH: Tumor Necrosis Factor-alpha Immunology Neurology (clinical) 030217 neurology & neurosurgery Interleukin-1 |
| Zdroj: | Journal of NeuroVirology Journal of NeuroVirology, Springer Verlag, 2000, 6 (4), pp.350-357. ⟨10.3109/13550280009030761⟩ |
| ISSN: | 1538-2443 1355-0284 |
| DOI: | 10.3109/13550280009030761 |
| Popis: | International audience; HTLV-1 is the causative agent of a chronic neurological disease, TSP/HAM. The persistently activated CTL response to the viral protein Tax-1 suggests the existence of persistent viral replication with continuous expression of Tax-1. Although CD4+ T-cell is the main target for HTLV-1, previous observations have indicated that the astrocyte, the major neural cell in close contact with blood vessel and thus with HTLV-1-infected T-cells infiltrating the CNS, may also be infected. We tested in vitro the hypothesis of persistent/restricted infection in human and rat astrocytes after transient contact with an infectious T-cell line (C91PL). Long-term analysis showed prolonged expression of Tax-1 in astrocytes, associated with secretion of inflammatory mediators (TNFalpha, IL1alpha, MMP-2, and MMP-9). These data suggest a possible contribution of Tax-1-expressing astrocytes to TSP/HAM pathogenesis. |
| Databáze: | OpenAIRE |
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