Effects of hydroxyl radical scavengers on relaxation of supercoiled DNA by aminomethyl-trimethyl-psoralen and monochromatic UVA photons
| Autor: | Asha A. Oroskar, Christopher R. Lambert, Meyrick J. Peak |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Azides
Formates Ultraviolet Rays medicine.medical_treatment Radical Acetates Photochemistry Biochemistry chemistry.chemical_compound Physiology (medical) medicine Mannitol Trioxsalen Formate Sodium Azide Psoralen Acetic Acid Photosensitizing Agents DNA Superhelical Hydroxyl Radical Free Radical Scavengers chemistry DNA supercoil Sodium azide Hydroxyl radical Azide Plasmids |
| Zdroj: | Free Radical Biology and Medicine. 20:751-756 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/0891-5849(95)02158-2 |
| Popis: | The ability of scavengers of hydroxyl radical (OH radical) to modulate the photosensitized relaxation (induction of the first single-strand break) of supercoiled plasmid DNA with UVA photoactivated 4'-aminomethyl-4,5',8-trimethylpsoralen was examined by comparing the dose reduction factor (DRF: the ratio of fluence required to induce the same degree of relaxation in the absence to the presence of OH radical scavengers). The addition of mannitol, azide, acetate, or formate at concentrations inversely proportional to the value of the rate constants for the scavenging of OH radicals partially attenuated the supercoiled DNA relaxation. The degrees of protection afforded by the four scavengers in the presence of AMT photoactivated by either 334 nm or 365 nm monochromatic photons were similar, giving an average DRF of about 0.25 in all cases. Given the diverse chemical nature of the scavengers and their wide range of concentrations utilized, these findings are evidence for the involvement of a Type I photosensitization in the induction of DNA single-strand breaks by photoactivated AMT. |
| Databáze: | OpenAIRE |
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