Helicobacter marmotae and novel Helicobacter and Campylobacter species isolated from the livers and intestines of prairie dogs
| Autor: | James G. Fox, Zeli Shen, Melissa W. Mobley, Floyd E. Dewhirst, Nancy S. Taylor, Maike Beisele, Ellen M. Buckley, M. Z. Abedin, Nicola Parry |
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| Přispěvatelé: | Massachusetts Institute of Technology. Division of Comparative Medicine, Fox, James G., Shen, Zeli, Beisele, Maike, Mobley, Melissa W., Taylor, Nancy S., Buckley, Ellen Marie, Parry, Nicola Maria Anne |
| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Male Microbiology (medical) Molecular Sequence Data Prairie dog Biology medicine.disease_cause DNA Ribosomal Polymerase Chain Reaction Microbiology Helicobacter Infections law.invention law Helicobacter RNA Ribosomal 16S biology.animal Campylobacter Infections medicine Animals Polymerase chain reaction Models of Infection Campylobacter Hepatobiliary disease Sciuridae Sequence Analysis DNA General Medicine Ribosomal RNA biology.organism_classification 16S ribosomal RNA Intestines Cynomys ludovicianus Liver Female |
| Zdroj: | Prof. Fox via Howard Silver |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Prairie dogs (Cynomys ludovicianus) are used to study the aetiology and prevention of gallstones because of the similarities of prairie dog and human bile gallstone composition. Epidemiological and experimental studies have suggested a connection between infection with Helicobacter species and cholesterol cholelithiasis, cholecystis and gallbladder cancer. Ten of the 34 prairie dogs in this study had positive Helicobacter species identified by PCR using Helicobacter genus-specific primers. Ten of 34 prairie dogs had positive Campylobacter species identified in the intestine by PCR with Campylobacter genus-specific primers. Six Helicobacter sp. isolates and three Campylobacter sp. isolates were identified taxonomically by 16S rRNA gene analysis. The prairie dog helicobacters fell into three clusters adjacent to Helicobacter marmotae. On the basis of 16S rRNA gene sequence analysis, three strains in two adjacent clusters were included in the species H. marmotae. Three strains were only 97.1 % similar to the sequence of H. marmotae and can be considered a novel species with the provisional designation Helicobacter sp. Prairie Dog 3. The prairie dog campylobacters formed a single novel cluster and represent a novel Campylobacter sp. with the provisional designation Campylobacter sp. Prairie Dog. They branched with Campylobacter cuniculorum at 96.3 % similarity and had the greatest sequence similarity to Campylobacter helveticus at 97.1 % similarity. Whether H. marmotae or the novel Helicobacter sp. and Campylobacter sp. identified in prairie dogs play a role in cholesterol gallstones or hepatobiliary disease requires further studies. National Institutes of Health (U.S.) (grant R01CA067529) National Institutes of Health (U.S.) (grant R01DK052413) National Institutes of Health (U.S.) (grant T32RR007036) National Institutes of Health (U.S.) (grant P30ES002109) National Institutes of Health (U.S.) (grant R01DK070865) National Institutes of Health (U.S.) (grant P01CA026731) |
| Databáze: | OpenAIRE |
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