Zamilon, a novel virophage with Mimiviridae host specificity
| Autor: | Didier Raoult, Bernard La Scola, Mondher Boughalmi, Olivier Croce, Samia Benamar, Isabelle Pagnier, Morgan Gaia, Philippe Colson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Virophages
Science Genome Viral Nucleocytoplasmic large DNA viruses Microbiology Host Specificity Virology Genetics Mimiviridae Evolutionary Systematics Bacteriophages Molecular Biology Techniques Sequencing Techniques Molecular Biology Mimivirus Evolutionary Biology Multidisciplinary Sequence Assembly Tools biology Virophage Biology and Life Sciences Computational Biology Sputnik virophage Genomics biology.organism_classification Genome Analysis Viral Replication Phylogenetics Co-Infections Satellite Viruses Medicine Phycodnaviridae Cafeteria roenbergensis virus Sequence Analysis Viral Transmission and Infection Research Article |
| Zdroj: | PLoS ONE, Vol 9, Iss 4, p e94923 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Virophages, which are potentially important ecological regulators, have been discovered in association with members of the order Megavirales. Sputnik virophages target the Mimiviridae, Mavirus was identified with the Cafeteria roenbergensis virus, and virophage genomes reconstructed by metagenomic analyses may be associated with the Phycodnaviridae. Despite the fact that the Sputnik virophages were isolated with viruses belonging to group A of the Mimiviridae, they can grow in amoebae infected by Mimiviridae from groups A, B or C. In this study we describe Zamilon, the first virophage isolated with a member of group C of the Mimiviridae family. By co-culturing amoebae with purified Zamilon, we found that the virophage is able to multiply with members of groups B and C of the Mimiviridae family but not with viruses from group A. Zamilon has a 17,276 bp DNA genome that potentially encodes 20 genes. Most of these genes are closely related to genes from the Sputnik virophage, yet two are more related to Megavirus chiliensis genes, a group B Mimiviridae, and one to Moumouvirus monve transpoviron. |
| Databáze: | OpenAIRE |
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