Differential expression of groEL-1, incB, pyk-F, tal, hctA and omcB genes during Chlamydia trachomatis developmental cycle

Autor: Willem A. Sturm, Sinaye Ngcapu, Gugulethu F. Mzobe, Bronwyn C. Joubert
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Keratinocytes
Time Factors
Cellular differentiation
Cultured tumor cells
Gene Expression
Chlamydia trachomatis
medicine.disease_cause
Pathology and Laboratory Medicine
Epithelium
Chlamydia Infection
Medical Conditions
Animal Cells
Gene expression
Medicine and Health Sciences
Chlamydia
Regulation of gene expression
Multidisciplinary
Cell Differentiation
Bacterial Pathogens
DNA-Binding Proteins
Infectious Diseases
Medical Microbiology
Cell lines
Medicine
Pathogens
Cellular Types
Anatomy
Biological cultures
Research Article
Bacterial Outer Membrane Proteins
Science
030106 microbiology
Pyruvate Kinase
Sexually Transmitted Diseases
Biology
Microbiology
Cell Line
03 medical and health sciences
Bacterial Proteins
medicine
Genetics
Humans
Gene Regulation
HeLa cells
Gene
Microbial Pathogens
Transcription Activator-Like Effectors
Bacteria
Organisms
Biology and Life Sciences
Epithelial Cells
Cell Biology
Chaperonin 60
Gene Expression Regulation
Bacterial
medicine.disease
Cell cultures
Phosphoproteins
Molecular biology
In vitro
Research and analysis methods
030104 developmental biology
Biological Tissue
Cell culture
Developmental Biology
Zdroj: PLoS ONE, Vol 16, Iss 4, p e0249358 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Chlamydia trachomatis infects squamous and columnar epithelia at the mucosal surface. Research on gene expression patterns of C. trachomatis has predominantly focused on non-native host cells, with limited data on growth kinetics and gene expression of chlamydia in keratinocytes. Here, we investigated whether early, mid, and late chlamydial genes observed in HeLa cell line studies were co-ordinately regulated at the transcriptional level even in the keratinized cell line model and whether the expression was stage-specific during the developmental cycle. HaCaT cell lines were infected with chlamydia clinical isolates (US151and serovar E) and reference strain (L2 434). Expression of groEL-1, incB, pyk-F, tal, hctA, and omcB genes was conducted with comparative real-time PCR and transcriptional events during the chlamydial developmental cycle using transmission electron microscopy. The relative expression level of each gene and fold difference were calculated using the 2-ΔΔCT method. The expression of groEL-1 and pyk-F genes was highest at 2 hours post-infection (hpi) in the L2 434 and serovar E. The expression of incB gene increased at 2 hpi in L2 434 and serovar E but peaked at 12 hpi in serovar E. L2 434 and US151 had similar tal expression profiles. Increased expression of hctA and omcB genes were found at 2 and 36 hpi in L2 434. Both clinical isolates and reference strains presented the normal chlamydial replication cycle comprising elementary bodies and reticulate bodies within 36 hpi. We show different gene expression patterns between clinical isolates and reference strain during in vitro infection of keratinocytes, with reference strain-inducing consistent expression of genes. These findings confirm that keratinocytes are appropriate cell lines to interrogate cell differentiation, growth kinetics, and gene expression of C. trachomatis infection. Furthermore, more studies with different clinical isolates and genes are needed to better understand the Chlamydial pathogenesis in keratinocytes.
Databáze: OpenAIRE
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