Sequence analysis of a parvovirus B19 isolate and baculovirus expression of the non-structural protein
| Autor: | K. E. Hicks, B. J. Cohen, R. C. N. Cubel, J. P. Clewley |
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| Rok vydání: | 1996 |
| Předmět: |
Sequence analysis
viruses Molecular Sequence Data Genome Viral Spodoptera Viral Nonstructural Proteins Antibodies Viral law.invention Serology Capsid Antigen law Virology Parvovirus B19 Human Animals Parvoviridae Base Sequence biology Parvovirus Nucleic acid sequence virus diseases General Medicine biology.organism_classification Molecular biology Recombinant Proteins Recombinant DNA Capsid Proteins Baculoviridae |
| Zdroj: | Archives of Virology. 141:1319-1327 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01718833 |
| Popis: | Serology for parvovirus B19 has been hampered by limited availability of antigen which has often had to be isolated from viraemic blood donations. We have determined the sequence of the genome of one such isolate (Stu). It is 99% similar to the sequences of two other isolates (Wi and Au) except at the far 5'-end, where it is more similar to the terminus of another isolate (Ala/Alb). Recombinant nonstructural protein, NS, was constructed. Antibodies to NS, as well as to the capsid proteins, VP1/2, were detected in patients with B19 infection. |
| Databáze: | OpenAIRE |
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