Clinical demonstration of isolation of Nocardia asteroides on buffered charcoal-yeast extract media
| Autor: | Victor L. Yu, R M Vickers, J D Rihs |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Microbiology (medical) food.ingredient Nocardia Infections Microbiology food Yeast Dried medicine Humans Yeast extract Agar Lung Diseases Fungal biology Sputum Nocardia Middle Aged biology.organism_classification Isolation (microbiology) Nocardiaceae Culture Media respiratory tract diseases Charcoal Nocardia asteroides Actinomycetales medicine.symptom Research Article |
| Zdroj: | Journal of Clinical Microbiology. 30:227-228 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.30.1.227-228.1992 |
| Popis: | Nocardia asteroides was isolated only from sputum samples, obtained from three patients with pulmonary nocardiosis, that had been cultured onto buffered charcoal-yeast extract (BCYE) and selective BCYE media as part of laboratory workups for Legionella species. A decontamination procedure with low-pH pretreatment (KCl-HCl solution) had been performed on the sputa prior to culture onto the BCYE media because direct cultures on the media were overgrown with commensal microflora. Chalky white colonies, 0.5 to 1.0 mm in diameter, that were subsequently identified as N. asteroides grew well on the BCYE media. Thus, the techniques and the selective media used for Legionella species were useful for isolating Nocardia species from sputum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|