Is remaining intervertebral disc tissue interfering with bone generation during fusion of two vertebrae?

Autor: Albert G. Veldhuizen, Roel Kuijer, Sjoerd K. Bulstra, Charles M. M. Peeters, D. Kok, D. L. M. Oterdoom, Z. Mardina, F. H. Wapstra
Přispěvatelé: Public Health Research (PHR), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Physiology
medicine.medical_treatment
Intervertebral Disc Degeneration
Pathology and Laboratory Medicine
Extracellular matrix
Nervous System Procedures
Animal Cells
Osteogenesis
Immune Physiology
Medicine and Health Sciences
Enzyme assays
Colorimetric assays
Intervertebral Disc
Bioassays and physiological analysis
Immune Response
Cells
Cultured
Connective Tissue Cells
Innate Immune System
Multidisciplinary
MTT assay
Chemistry
Stem Cells
Cell Differentiation
musculoskeletal system
Osteoblast Differentiation
Extracellular Matrix
Mitochondria
medicine.anatomical_structure
Connective Tissue
Spinal fusion
Medicine
Cytokines
Cellular Types
Anatomy
Research Article
musculoskeletal diseases
Science
Immunology
Primary Cell Culture
Surgical and Invasive Medical Procedures
Proinflammatory cytokine
Andrology
Signs and Symptoms
Diagnostic Medicine
medicine
Humans
Inflammation
Osteoblasts
Mesenchymal stem cell
Biology and Life Sciences
Intervertebral disc
Mesenchymal Stem Cells
Cell Biology
Molecular Development
In vitro
Coculture Techniques
Research and analysis methods
Biological Tissue
Spinal Fusion
Cell culture
Immune System
Biochemical analysis
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, 14(4):e0215536. PUBLIC LIBRARY SCIENCE
PLoS ONE, Vol 14, Iss 4, p e0215536 (2019)
ISSN: 1932-6203
Popis: Study designlaboratory research.BackgroundThrough the increasing number of minimally invasive procedures in spinal fusion surgery, the complete removal of intervertebral disc (IVD) tissue has become more a challenge. Remaining IVD may interfere with the biological process of bone formation.ObjectiveIn order to establish whether complete removal of IVD tissue will improve or inhibit the fusion process, the effects of different concentrations of extracts of inflamed disc tissue on the mitochondrial activity of mesenchymal stem cells (MSCs), and the capacity to mineralize their extracellular matrix by osteoblasts and differentiated MSCs were tested in vitro.MethodsA MTT assay was conducted to measure the mitochondrial activity of MSCs, and an Alizarin Red S staining quantification assay to measure the deposition of calcium by osteoblasts and differentiated, bone marrow-derived MSCs.ResultsA significantly higher mitochondrial activity was shown in MSCs co-cultured with extracts of IVD tissue (10%, 50%, and 100%) compared with the control group after 48 hours of incubation, indicating that the IVD tissue extracts stimulated the mitochondrial activity of MSCs. This effect appeared to be inversely proportional to the concentration of IVD tissue extract. No significant differences in mineralization by human osteoblasts or differentiated MSCs were found between the samples incubated with IVD tissue extracts (3% and 33%) and the control samples.ConclusionOur findings indicate that remaining IVD tissue has more of a stimulating than inhibiting effect on the activity of MSCs. Even if inflammatory cytokines are produced, these do not result in a net inhibition of cellular activity or osteogenic differentiation of MSCs.
Databáze: OpenAIRE
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