Extraction of skin test activity from Coccidioides immitis mycelia by water, perchloric acid, and aqueous phenol extraction
| Autor: | R W Wheat, E R Scheer, K S Su Chung, E P Ornellas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1978 |
| Předmět: |
Antigens
Fungal Immunology Guinea Pigs Biology Microbiology chemistry.chemical_compound Phenols Phenol Animals Coccidioides Perchloric acid Skin Tests Glucosamine Aqueous solution Chromatography Perchlorates Extraction (chemistry) Phenol extraction Water biology.organism_classification Infectious Diseases Glucose Biochemistry chemistry Solubility Parasitology Specific activity Mannose Research Article |
| Popis: | Water, perchloric acid extracts, and fractions of partially defatted whole mycelia of Coccidioides immitis contained delayed skin test activity when tested in C. immitis-infected guinea pits. Aqueous phenol extraction of these fractions resulted in partitioning of activity between aqueous-soluble and phenol-soluble fractions; activity was found to be water soluble after removal of phenol by extensive dialysis. Highest specific activity skin test antigen was invariably found in the phenol-soluble phase, water-soluble fraction. Material of equivalent activity could also be extracted directly from the defatted mycelia. Skin test active fractions contained glucose, mannose, 3-O-methylmannose, glucosamine, and amino acids. |
| Databáze: | OpenAIRE |
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