FYNis overexpressed in human prostate cancer
| Autor: | Carrie W. Rinker-Schaeffer, Walter M. Stadler, Kenneth J. Pienta, Javed Siddiqui, Ramasamy Jagadeeswaran, Hikmat Al-Ahmadie, Ravi Salgia, Edwin M. Posadas, Victoria L. Robinson, Kristen Otto, Kristen Kasza, Maria Tretiakov |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Urology Blotting Western Proto-Oncogene Proteins c-fyn Cell morphology Article Prostate cancer FYN LNCaP Humans Medicine Paxillin Aged Prostatic Intraepithelial Neoplasia biology Reverse Transcriptase Polymerase Chain Reaction Kinase business.industry Prostatic Neoplasms Cancer Middle Aged medicine.disease Immunohistochemistry Up-Regulation Gene Expression Regulation Neoplastic Case-Control Studies Focal Adhesion Kinase 1 biology.protein Cancer research biological phenomena cell phenomena and immunity business Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | BJU International. 103:171-177 |
| ISSN: | 1464-410X 1464-4096 |
| DOI: | 10.1111/j.1464-410x.2008.08009.x |
| Popis: | OBJECTIVE To test the hypothesis that FYN, a member of the SRC family of kinases (SFKs), is up-regulated in prostate cancer, as FYN is functionally distinct from other SFKs, and interacts with FAK and paxillin (PXN), regulators of cell morphology and motility. MATERIALS AND METHODS Through data-mining in Oncomine (http://www.oncomine.org), cell-line profiling with immunoblotting, quantitative reverse transcription and polymerase chain reaction (RT-PCR) and immunohistochemical analysis, we described FYN expression in prostate cancer. The analysis included 32 cases of prostate cancer, nine of prostatic intraepithelial neoplasia (PIN) and 19 normal prostates. Samples were scored for the percentage of stained glands and intensity of staining (from 0 to 3). Each sample was assigned a composite score generated by multiplying percentage and intensity. RESULTS Data-mining showed an eight times greater FYN expression in prostate cancer than in normal tissue; this was specific to FYN and not present for other SFKs. Expression of FYN in prostate cancer cell lines (LNCaP, 22Rv1, PC3, DuPro) was detected using quantitative RT-PCR and immunoblotting. Expression of FYN and its signalling partners FAK and PXN was detected in human tissue. Comparing normal with cancer samples, there was a 2.1-fold increase in median composite score for FYN (P |
| Databáze: | OpenAIRE |
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