Infection of Owl Monkeys (Aotus trivirgatus) and Cynomolgus Monkeys (Macaca fascicularist) with Hepatitis E Virus from Mexico
| Autor: | John Ticehurst, Jeffrey D. Caudill, Leonard N. Binn, Charles H. Hoke, Walter F. Engler, Ludmila V.S. Asher, Maria H. Sjogren, James W. LeDuc, Timothy L. Mensing, Krzysztof Krawczynski, Daniel W. Bradley, Lowrey Rhodes |
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| Rok vydání: | 1992 |
| Předmět: |
viruses
Fluorescent Antibody Technique Biology Virus Replication medicine.disease_cause Virus Serology Feces Hepatitis E virus Antigen parasitic diseases Aotus trivirgatus medicine Animals Bile Immunology and Allergy Hepatitis Antibodies Microscopy Immunoelectron Antigens Viral Mexico Hepatitis Virion virus diseases Alanine Transaminase medicine.disease Virology digestive system diseases Hepatitis E Disease Models Animal Macaca fascicularis Infectious Diseases Liver Humoral immunity biology.protein Antibody |
| Zdroj: | Journal of Infectious Diseases. 165:835-845 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Owl and cynomolgus monkeys were inoculated with hepatitis E virus (HEV) to compare disease models and produce antibody and virus. By immune electron microscopy (IEM), all six owl monkeys were shown to have serologic responses manifested by unusually high levels of anti-HEV at 6 months, but only three developed hepatitis. Virus-related antigen in liver (HEV Ag) was detected by immunofluorescence microscopy of biopsies from two of four owl monkeys; one with HEV Ag also had HEV in acute-phase bile (detected by IEM) and feces (detected by infecting another owl monkey). In contrast, cynomolgus monkeys propagated HEV to higher levels and all five had hepatitis. Moderate-to-high levels of HEV Ag correlated with detectable HEV in bile for both species. Thus, the value of using HEV-infected cynomolgus was confirmed. Owl monkeys were shown to be HEV-susceptible and sources of high-level anti-HEV; Sustained anti-HEV in these monkeys may also be useful for understanding immune responses. |
| Databáze: | OpenAIRE |
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