Mechanism underlying inhibition of the capsaicin receptor-mediated current by pregnenolone sulfate in rat dorsal root ganglion neurons
| Autor: | Shu-Cheng Chen, Fong-Sen Wu |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Patch-Clamp Techniques Time Factors Receptors Drug TRPV1 Inhibitory postsynaptic potential Membrane Potentials Rats Sprague-Dawley chemistry.chemical_compound Dorsal root ganglion Ganglia Spinal medicine Animals Drug Interactions Molecular Biology Cells Cultured Neurons Dose-Response Relationship Drug General Neuroscience Neural Inhibition Rats medicine.anatomical_structure Nociception chemistry Capsaicin Pregnenolone Biophysics Neurology (clinical) Neuron Pregnenolone sulfate Neuroscience Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 1027:196-200 |
| ISSN: | 0006-8993 |
| Popis: | The effect of pregnenolone sulfate (PS) on the capsaicin receptor-mediated current was studied in acutely dissociated rat dorsal root ganglion neurons using the whole-cell voltage-clamp technique. PS acted extracellularly to inhibit the capsaicin-induced current in a not competitive but concentration-dependent manner, with an EC 50 of 13 μM and a maximal inhibition of 65%. Furthermore, antagonism of the capsaicin response by PS is neither voltage- nor agonist-dependent, indicating that PS did not act as an open-channel blocker. The inhibitory action of PS on the capsaicin-induced current may provide a basis for reducing capsaicin receptor-mediated nociception. |
| Databáze: | OpenAIRE |
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