Chronic vitamin D insufficiency impairs physical performance in C57BL/6J mice
| Autor: | Maria Rodríguez-Gonzalez, Bruce R. Troen, Merced M Leiker, Manhui Pang, Zachary Sheridan, Norma J. Nowak, Jonathan E. Bard, Kenneth L. Seldeen, Mireya Hernandez |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Muscle tissue Vitamin Aging medicine.medical_specialty muscle Population Muscle Proteins vitamin D 030209 endocrinology & metabolism sarcopenia Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Atrophy Bone Density Internal medicine medicine Vitamin D and neurology Animals Muscle Strength education Inflammation education.field_of_study SKP Cullin F-Box Protein Ligases microRNA Bone Density Conservation Agents business.industry Vitamins Cell Biology Vitamin D Deficiency medicine.disease mitochondria Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Endocrinology Gene Expression Regulation chemistry Physical performance Sarcopenia Body Composition Lean body mass Cytokines business Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| Popis: | Vitamin D insufficiency (serum 25-OH vitamin D < 30 ng/ml) affects 70-80% of the general population, yet the long-term impacts on physical performance and the progression of sarcopenia are poorly understood. We therefore followed 6-month-old male C57BL/6J mice (n=6) consuming either sufficient (STD, 1000 IU) or insufficient (LOW, 125 IU) vitamin D3/kg chow for 12 months (equivalent to 20-30 human years). LOW supplemented mice exhibited a rapid decline of serum 25-OH vitamin D levels by two weeks that remained between 11-15 ng/mL for all time points thereafter. After 12 months LOW mice displayed worse grip endurance (34.6 ± 14.1 versus 147.5 ± 50.6 seconds, p=0.001), uphill sprint speed (16.0 ± 1.0 versus 21.8 ± 2.4 meters/min, p=0.0007), and stride length (4.4 ± 0.3 versus 5.1 ± 0.3, p=0.002). LOW mice also showed less lean body mass after 8 months (57.5% ± 5.1% versus 64.5% ± 4.0%, p=0.023), but not after 12 months of supplementation, as well as greater protein expression of atrophy pathway gene atrogin‑1. Additionally, microRNA sequencing revealed differential expression of mIR‑26a in muscle tissue of LOW mice. These data suggest chronic vitamin D insufficiency may be an important factor contributing to functional decline and sarcopenia. |
| Databáze: | OpenAIRE |
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